Monospecific Direct Coombs IgG, Blood
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Detecting antibodies bound to RBC
Investigation of hemolytic anemia
Direct Antiglobulin Test Using Standard AABB Techniques
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Monospecific Direct Coombs IgG, B
Second ID 32822, Location DTL
Second ID 32823, Location DTL
Second ID 32823, Location DTL
Specimen Type Describes the specimen type needed for testing
Whole Blood EDTA
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen must arrive within 72 hours of draw.
Container/Tube: Pink top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Send specimen in original tube.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Whole Blood EDTA||Ambient (preferred)||48 hours|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
IgG antibody may be present on patient or donor (transfused) RBCs and may cause hemolysis. The antibodies may be directed against self-antigens (autoimmune hemolysis), maternal antigens (hemolytic disease of the newborn), donor antigens (eg, alloimmune transfusion reaction), or drugs.
The presence of in vivo coating of RBC with IgG can be demonstrated by the direct antiglobulin (Coombs) test.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
If positive, reaction is graded (positive 1+ to 4+).
The presence or absence of IgG is used in conjunction with other testing and clinical data to aid in the characterization of Hemolysis as immune-mediated. Possible causes include autoimmune hemolytic anemia, drug-induced hemolysis, hemolytic disease of the newborn, and alloimmune reactions to recently transfused RBC.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements.
Method Description Describes how the test is performed and provides a method-specific reference
Monospecific antihuman globulin reacts with red cell-bound complement to produce agglutination. (AABB Technical Manual. 14th edition. Edited by V Vengelen-Tyler. Bethesda, MD, AABB, 2002, pp 258-265)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|MDCG||Monospecific Direct Coombs IgG, B||1006-6|