Protein, Total, Random, Urine
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Evaluation of renal disease
Screening for monoclonal gammopathy
Dye Binding (Pyrogallol Red)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Protein, Total, Random, U
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Container/Tube: Plastic, 6-mL tube (Supply T465)
Specimen Volume: 5 mL
1. Collect a random urine specimen.
2. No preservative.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Urine||Refrigerated (preferred)||14 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Protein in urine is normally composed of a combination of plasma-derived proteins that have been filtered by glomeruli and have not been reabsorbed by the proximal tubules and proteins secreted by renal tubules or other accessory glands.
Increased amounts of protein in the urine may be due to:
-Glomerular proteinuria: caused by defects in permselectivity of the glomerular filtration barrier to plasma proteins (eg, glomerulonephritis or nephrotic syndrome)
-Tubular proteinuria: caused by incomplete tubular reabsorption of proteins (eg, interstitial nephritis)
-Overflow proteinuria: caused by increased plasma concentration of proteins (eg, multiple myeloma, myoglobinuria)
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
2-17 years: 0.0-0.10 mg/mg
18-65 years: 0.0-0.04 mg/mg
Reference values have not been established for patients less than 2 years or greater than 65 years of age.
Total protein >500 mg/24 hours should be evaluated by immunofixation to determine if a monoclonal immunoglobulin light chain is present, and if so, identify it as either kappa or lambda type.
Urinary protein levels may rise to 300 mg/24 hours in healthy individuals after vigorous exercise.
Low-grade proteinuria may be seen in inflammatory or neoplastic processes involving the urinary tract.
In a random urine specimen, a protein/creatinine or protein/osmolality ratio can be used roughly to approximate 24-hour excretion rates. The normal protein-to-creatinine ratio for adults 18 to 65 years is < or =0.04 mg/mg creatinine and the normal protein-to-osmolality ratio is <0.12.(1) For children 2 to 17 years, the normal protein-to-creatinine ratio is < or =0.1 mg/mg creatinine and protein/osmolality ratio is <0.15 Kg H2O/mosm/L.(2)
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
False-positive urine protein levels (increased) may be due to contamination of urine with menstrual blood, prostatic secretions, or semen.
Protein electrophoresis and immunofixation may be required to characterize and interpret the proteinuria.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Wilson DM, Anderson RL: Protein-osmolality ratio for the quantitative assessment of proteinuria from a random urinalysis sample. Am J Clin Pathol 1993 Oct;100(4):419-424
2. Morgenstern BZ, Butani L, Wollan P, et al: Validity of protein-osmolality versus protein-creatinine ratios in the estimation of quantitative proteinuria from random samples of urine in children. Am J Kidney Dis 2003 Apr;41(4):760-766
3. Keren DF: Clinical indications for electrophoresis and immunofixation. In Manual of Clinical Laboratory Immunology. Fifth edition. Edited by NR Rose, E Conway de Macario, JD Folds, et al. Washington, DC, ASM Press, 1997, pp 65-74
Method Description Describes how the test is performed and provides a method-specific reference
This assay uses colormetric dye binding on an automated platform. Pyrogallol red dye is combined with molybdenum acid forming a red complex with maximum absorption at 470 nm. When this complex is combined with protein under acidic conditions, its maximum absorption is shifted to a longer wavelength and develops a blue-purple color with a maximum at 604 nm. The concentration of protein in the specimen is equivalent to the absorbance of the dye urine mixture measured at 600 nm.(Package insert: Wako)
The enzymatic method is based on the determination of sarcosine from creatinine with the aid of creatininase, creatinase, and sarcosine oxidase. The liberated hydrogen peroxide is measured via a modified Trinder reaction using a colorimetric indicator. Optimization of the buffer system and the colorimetric indicator enables the creatinine concentration to be quantified both precisely and specifically.(Package insert: Roche Diagnostics, Indianapolis IN, 2004)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Same day/1 day
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|PTCON||Protein, Total, Random, U||2888-6|