Test ID: FS
Fungal Smear
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Detection of fungi in clinical specimens
Method Name A short description of the method used to perform the test
Calcofluor/KOH Stain
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen must arrive within 24 hours of collection.
Container/Tube: Sterile container
Specimen Volume: Entire collection
Collection Instructions: Collect a raw specimen.
Additional Information: Specimen source is required.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Fixed tissue; viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth); specimen source of environmental; wood shaft or charcoal swab |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Many fungi in the environment cause disease in severely compromised human hosts. Accordingly, the range of potential pathogenic fungi has increased as the number of immunosuppressed individuals (persons with AIDS, patients receiving chemotherapy or transplant rejection therapy, etc.) has increased.
Few fungal diseases can be diagnosed clinically; most are diagnosed by isolating and identifying the infecting fungus in the clinical laboratory.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
Interpretation Provides information to assist in interpretation of the test results
Positive slides are reported as 1 or more of the following: yeast or hyphae present, organism resembling Blastomyces dermatitidis, Histoplasma capsulatum, Coccidioides immitis, Cryptococcus neoformans, or Malassezia furfur.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
LaRocco MT: Reagents, stains, and media: mycology. In Manual of Clinical Microbiology. 8th edition. Washington, DC, ASM Press, 2003, pp 1686-1692
Method Description Describes how the test is performed and provides a method-specific reference
Calcofluor white, a fluorescent textile brightener, nonspecifically binds with chitin in the cell wall of fungi. Examination of stained specimens using fluorescent microscopy allows for the detection of fungi due to the fluorescence of calcofluor white present on the fungal cell wall. Potassium hydroxide is added to hasten clearing of viscous specimens, and Evans blue is added to prevent nonspecific fluorescence. (Merz WG, Roberts GD: Detection and recovery of fungi in clinical specimens. In Manual of Clinical Microbiology. 9th edition. Edited by PR Murray, EJ Baron, MA Pfaller, et al. Washington DC, ASM Press, 1995)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
87210
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| FS | Fungal Smear | In Process |
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