Test ID: ANAE
Bacterial Culture, Anaerobic
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Diagnosing anaerobic bacterial infections
Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
When this test is ordered, the reflex tests may be performed and charged.
Method Name A short description of the method used to perform the test
Conventional Culture Techniques
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen should arrive within 24 hours of collection.
Specimen Type: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds
Container/Tube: Anaerobic transport vial (BBL Port-A-Cul Vial) (Supply T591)
Collection Instructions: Specimen should be obtained by using a needle and syringe from a source not normally colonized by anaerobes.
Additional Information: Specimen source is required.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Swab |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | 72 hours |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Anaerobic bacteria are the greatest component of the human body's normal bacterial flora colonizing the skin, oral cavity, and genitourinary and lower gastrointestinal tracts. Their presence is important in preserving vitamin and other nutrient absorption and in preventing infection with pathogenic bacteria.
Anaerobes generally are of low pathogenicity but may possess virulence factors such as endotoxin or polysaccharide capsules or produce extracellular toxins. Disease occurs when a large inoculum develops in an area lacking oxygen and/or poor blood supply.
Typical anaerobic infections include periodontitis, abdominal or pelvic abscesses, endometritis, pelvic inflammatory disease, aspiration pneumonia, empyema and lung abscesses, sinusitis, brain abscesses, gas gangrene, and other soft tissue infections. Many Bacterioides produce beta-lactamase and are resistant to penicillins and cephalosporins. Imipenem, metronidazole, and clindamycin are effective agents although resistance to clindamycin is increasing.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Not applicable
Interpretation Provides information to assist in interpretation of the test results
Isolation of anaerobes in significant numbers from well collected specimens including blood, other normally sterile body fluids, or closed collections of purulent fluid indicates infection with that (those) organism(s).
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Specimens should be collected by needle and syringe aspiration or surgical drainage to avoid contamination with normal-flora anaerobes; such contamination would make interpretation of culture results impossible.
Specimens must be transported in anaerobic transport vials.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Finegold SM, George WL: Anaerobic Infections in Humans. San Diego, CA, Academic Press, 1989
Method Description Describes how the test is performed and provides a method-specific reference
Appropriate specimens are inoculated onto blood agar, phenylethyl alcohol agar, and lysed blood agar containing gentamicin and vancomycin and into thioglycollate broth tubes which are incubated anaerobically for 48 hours. Colonies are subcultured to determine aerotolerance, and obligately anaerobic organisms may be identified by Gram stain and/or use of various differential media, biochemical tests, gas-liquid chromatographic analysis of volatile fatty acids produced during fermentation of glucose, and 16S RNA sequencing.(Koneman EW, Allen SD, Janda WM, et al: Color Atlas and Textbook of Diagnostic Microbiology. Fifth edition. New York, Lippincott-Raven Publishers, 1997, pp 709-784)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
Bacterial Culture, Anaerobic
87075
Anaerobe Ident
87076 (if appropriate)
Anaerobe with Chromatography Ident
87076 (if appropriate)
Anaerobe Ident by Sequencing
87153 (if appropriate)
Ident by MALDI-TOF mass spec
87077 (if appropriate)
Tissue Processing
87176 (if appropriate)
Anaerobe Suscep per agent
87181 (if appropriate)
Beta Lactamase
87185 (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| ANAE | Bacterial Culture, Anaerobic | In Process |
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