Test ID: HDCH
Cholesterol, HDL, Serum
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Cardiovascular risk assessment
Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test
Method Name A short description of the method used to perform the test
Enzymatic Colorimetric
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
HDL (High Density Lipoprotein),Cholesterol
HDL Cholesterol (order if only HDL indicated)
HDL Direct
High Density Lipoprotein, Cholesterol
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 7 days |
| Frozen | 30 days |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
High density lipoprotein (HDL) is the smallest of the lipoprotein particles and comprises a complex family of lipoprotein particles that exist in a constant state of dynamic flux as the particles interact
with other HDL particles and with low density lipoprotein (LDL) particles and very low density lipoprotein (VLDL) particles. HDL has the largest proportion of protein relative to lipid compared to
other lipoproteins (>50% protein).
Total cholesterol levels have long been known to be related to coronary heart disease (CHD). HDL cholesterol is also an important tool used to assess an individual's risk of developing CHD since a strong negative relationship between HDL cholesterol concentration and the incidence of CHD has been reported.
In some individuals, exercise increases the HDL cholesterol level; those with more physical activity have higher HDL cholesterol values.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
The National Cholesterol Education Program (NCEP) has set the following guidelines for lipids (total cholesterol, triglycerides, high density lipoprotein [HDL], and low density lipoprotein [LDL] cholesterol) in adults ages 18 and up:
HDL CHOLESTEROL
Low (removed HDL): <40 mg/dL
Normal: 40-60 mg/dL
High: >60 mg/dL
The National Cholesterol Education Program (NCEP) and National Health and Nutrition Examination Survey (NHANES) has set the following guidelines for lipids (total cholesterol, triglycerides, HDL, and LDL cholesterol) in children ages 2-17:
HDL CHOLESTEROL
Low HDL: <40 mg/dL
Borderline low: 40-59 mg/dL
Normal: > or =60 mg/dL
Also see age and sex adjusted reference values in Cholesterol, HDL-Percentile Ranking in Lipids and Lipoproteins in Blood Plasma (Serum) in Special Instructions.
Interpretation Provides information to assist in interpretation of the test results
Values >60 mg/dL are considered a negative risk factor for coronary heart disease (CHD) and are considered protective. Values > or = 80 to 100 mg/dL may indicate metabolic response to certain medications such as hormone replacement therapy, chronic liver disease, or some form of chronic intoxication, such as with alcohol, heavy metals, industrial chemicals including pesticides.
Values <40 mg/dL correlate with increased risk for CHD.
High density lipoprotein values < or = 5 mg/dL occur in Tangier disease, in association with cholestatic liver disease, and in association with diminished hepatocyte function.
See Lipids and Lipoproteins in Blood Plasma (Serum) in Special Instructions.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Fasting is not necessary but is preferable. However, if the high density lipoprotein cholesterol data is used to calculate the low density lipoprotein cholesterol, fasting is required since triglycerides concentration is used in the calculation.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Tietz Textbook of Clinical Chemistry. Edited by CA Burtis, ER Ashwood. Philadelphia, WB Saunders Company, 1994
2. Rifai N, Warnick GR: Laboratory Measurement of Lipids, Lipoproteins, and Apolipoproteins. AACC Press, Washington DC, 1994
Method Description Describes how the test is performed and provides a method-specific reference
Sulfated alpha-cyclodextrin forms water soluble complexes selectively with low density lipoprotein, very low density lipoprotein, and chylomicrons, and renders these lipoprotein particles resistant to the enzymatic activity of polyethylene glycol (PEG)-modified cholesterol esterase and cholesterol oxidase. PEG-modified cholesterol esterase selectively converts high density lipoprotein (HDL) cholesterol esters to free cholesterol, which, in the presence of O(2) reacts with PEG-cholesterol oxidase to produce delta-cholestenone and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide generated reacts with 4-aminophenazone and N-ethyl-N(3 methylphenyl)-N-succinyl ethylene diamine to form a quinone amine dye. The color intensity of this dye, measured photometrically at 600 nm, is proportional to the HDL-cholesterol concentration in the specimen. This method is referenced to the Centers of Disease Control and Prevention (CDC) standardized method performed in the Cardiovascular Risk Assessment Laboratory. (Package insert: Roche HDL-C Reagent, Roche Diagnostic Corp., Indianapolis, October 1999)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
83718
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| HDCH | Cholesterol, HDL, S | 2085-9 |
External
link
PDF
document
Excel
document
Word
document