Test ID: CCBF
Cell Count and Differential, Body Fluid
Secondary ID 
A test code used for billing and in test definitions created prior to November 2011
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
An aid in the diagnosis of joint disease, systemic disease, inflammation, malignancy, infection, and trauma
Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CCBF_ | Cell Count and Differential, BF | No | Yes |
| CRSF | Morphologic Review, BF | No | Yes |
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
When abnormal cytologic features are present, the laboratory may reflex to a miscellaneous cytology test. Fee codes for that test vary depending on review process.
Method Name A short description of the method used to perform the test
Automated or manual cell count/cytocentrifugation followed by manual differential and morphology review.
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
For Local Accounts Only
Sources: Synovial, pleural, peritoneal and pericardial
Specimen must arrive within 24 hours of collection.
Container/Tube:
Preferred: Body fluid container
Acceptable: EDTA or heparin
Specimen Volume: 1 mL
Additional Information: Indicate specimen source.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross OK |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Body Fluid | Ambient (preferred) | 24 hours |
| Refrigerated | 24 hours |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Body fluids, other than the commonly analyzed urine and blood, include synovial, pleural, peritoneal, and pericardial fluids. These fluids may be present in increased volumes and/or may contain increased numbers of normal and/or abnormal cells in a variety of disease states.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
TOTAL NUCLEATED CELLS:
Synovial fluid: <150/mcL
Peritoneal/pleural/pericardial fluid: <500/mcL
NEUTROPHILS:
Synovial Fluid: <25%
Peritoneal/pleural/pericardial fluid: <25%
LYMPHOCYTES:
Synovial fluid: <75%
MONOCYTES/MACROPHAGES:
Synovial fluid: 70%
Interpretation Provides information to assist in interpretation of the test results
Trauma and hemorrhage may result in increased red and white cells; red cells predominate. White blood cells are increased in inflammatory and infectious processes:
-Neutrophils predominate in bacterial infections
-Lymphocytes predominate in viral infections
-Macrophages may be increased in inflammatory and infectious processes
-Eosinophils may be increased in parasitic or fungal infections
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Kjeldsberg C, Knight J: Body Fluids: Laboratory examination of cerebrospinal, seminal, serous and synovial fluids. American Society of Clinical Pathologists, Chicago, 1993
2. Dyken PR, Shirley S, Trefz J, El Gammel T: Comparison of cyto-centrifugation and sedimentation techniques for CSF cyto-morphology. Acta Cytologica 1980;20:137-170
3. Sheth KV: Cerebrospinal and body fluid cell morphology through a hematologist's microscope, workshop presented at the ASCP-CAP Joint Spring Meeting, San Diego, March 1981
4. Schumacher AH, Reginato A: Atlas of Synovial Fluid Analysis and Crystal Identification. Lea and Febiger, Philadelphia; 1991
Method Description Describes how the test is performed and provides a method-specific reference
The cells are applied to a glass slide by cytocentrifugation. Wright-Giemsa stained slides are examined by light microscopy and a differential is performed. Total nucleated cell count is determined using an automated hematology analyzer or a microscopic counting chamber. (Instructions for Use: Sysmex XE-5000, Sysmex Corporation, Kobe, Japan, 2008)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
89051-Cell count with differential count
88108-Cytology for abnormal cells (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| FLD2 | Fluid Type | 14725-6 |
| OTH11 | Other Cells Are: | N/A |
| CMT81 | Comment | 48767-8 |
| APP2 | Gross Appearance | 9335-1 |
| TOT12 | Total Nucleated Cells | In Process |
| REV81 | Reviewed by: | In Process |
| RBC1 | Erythrocytes | 26455-6 |
| CMT37 | Comment | 48767-8 |
| NE_BF | Neutrophils | 26513-2 |
| LY_BF | Lymphocytes | 11031-2 |
| MM_BF | Monocytes/Macrophages | 30437-8 |
| EO_BF | Eosinophils | 26452-3 |
| BA_BF | Basophils | 28543-7 |
| OTH1 | Other Cells | N/A |
| CMT3 | Diff Comments | 48767-8 |
| DCCBF | Download CCBF | N/A |
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