NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
A screening aid in patients presenting with decreased high density lipid (HDL) cholesterol, to investigate potential hypoalphalipoproteinemia phenotypes prior to confirmatory molecular testing
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Fasting-overnight (12-14 hours)
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Mild OK; Gross OK
Mild OK; Gross OK
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Lysophosphatidylcholine (LPC), also referred to as lysolecithin, is the most abundant lysophospholipid in blood. LPCs may originate from the enzyme lecithin cholesterol acyltransferase (LCAT), by hepatic secretion or via phospholipase A2 activity. The framework of LPC species includes a glycerol frame, one fatty acid, and phosphocholine. The primary fatty acid species LPC associates with in plasma include palmitic acid (16:0) and stearic acid (18:0).(1) The 16:0 LPCs are enriched in HDL whereas the 18:0 forms are predominantly associated with the apolipoprotein-B-containing lipoproteins very-low-density lipoprotein (VDRL) and low-density lipoprotein (LDL). This association may reflect distinct metabolic pathways for individual LPC species.(2)
LPC is a major component of oxidized LDL which is thought to promote atherogenesis by increasing the chemotactic activity of monocytes, inducing adhesion molecules on endothelial cells and accumulating in ischemic myocardium.(3,4) Plasma LPC concentrations may be increased in individuals with various types of cancer and in non-insulin-dependent diabetes mellitus. Decreased concentrations of LPC may be seen in patients with hypoalphalipoproteinemia or acquired diseases such as renal failure.(5) Low LPC concentrations alone are not specific for LCAT deficiency. The LCATD / Lecithin Cholesterol Acyltransferase Deficiency Profile should be ordered if LCAT deficiency is suspected. Results of this profile only give a phenotypic interpretation and molecular testing is recommended for confirmation of LCAT deficiency.
LPC is also a source of lysophosphatidic acid (LPA) which is formed from LPC via hydrolysis. LPA has significant biological activities related to smooth muscle contraction, tumor cell invasion, and cell proliferation.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
> or =16 years: > or =62 mcmol/L
> or =16 years: > or =20 mcmol/L
Reference values have not been established for patients that are <16 years of age.
Decreased concentrations of 16:0 lysophosphatidylcholine (LPC) and 18:0 LPC may suggest lecithin cholesterol acyltransferase deficiency. Results must be interpreted in the context of the patient’s clinical presentation.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Croset M, Brossard N, Polette A, Lagarde M. Characterization of plasma unsaturated lysophosphatidylcholines in human and rat. Biochem J 2000; 345:61–67
2. Kontush A, Lhomme M, Chapman MJ. Unravelling the complexities of the HDL lipidome. J Lipid Res 2013 Nov;54(11):2950-2963
3. Quinn MT, Parthasarathy S, Steinberg D. Lysophosphatidylcholine: a chemotactic factor for human monocytes and its potential role in atherogenesis. Proc Natl Acad Sci USA 1988;85:2805–2809
4. Kume N, Cybulsky MI, Gimbrone MA. Lysophosphatidylcholine, a component of atherogenic lipoproteins, induces mononuclear leukocyte adhesion molecules in cultured human and rabbit arterial endothelial cells. J Clin Invest 1992; 90:1138-1144
5. Santamarina-Fojo S, Hoeg JM, Assman G, Brewer HB Jr: Lecithin cholesterol acyltransferase deficiency and fish eye disease. In The Metabolic and Molecular Basis of Inherited Disease. Eighth edition. Edited by C Scriver, A Beaudet, W Sly, et al. New York, McGraw-Hill Book Company, 2001, pp 2817-2833
Method Description Describes how the test is performed and provides a method-specific reference
Plasma is extracted with butanol after the addition of the internal standard (17:0 lysophosphatidylcholine [LPC]). After vortexing, centrifugation, and evaporation, the samples are reconstituted in methanol. Quantification is performed by selective reaction monitoring and positive mode electrospray liquid chromatography-tandem mass spectrometry (LC-MS/MS). (Lacey JM, Magera MJ, Matern D, et al: Rapid quantitative determination of lysophosphatidylcholine by liquid chromatography-tandem mass spectrometry [LC-MS/MS]. J Am Soc Mass Spec 2003;13S1:26S)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies; 11 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|83399||16:0 Lysophosphatidylcholine||In Process|
|23618||18:0 Lysophosphatidylcholine||In Process|