NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Investigating pancreatic disorders, usually pancreatitis
Colorimetric Rate Reaction
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Serum||Refrigerated (preferred)||21 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Lipases are enzymes that hydrolyze glycerol esters of long-chain fatty acids and produce fatty acids and 2-acylglycerol. Bile salts and a cofactor, colipase, are required for full catalytic activity and greatest specificity. The pancreas is the primary source of serum lipase. Both lipase and colipase are synthesized in the pancreatic acinar cells and secreted by the pancreas in roughly equimolar amounts. Lipase is filtered and reabsorbed by the kidneys. Pancreatic injury results in increased serum lipase levels.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
> or =16 years: 10-73 U/L
Reference values have not been established for patients that are <16 years of age.
In pancreatitis, lipase becomes elevated at about the same time as amylase (in 4-8 hours). But lipase may rise to a greater extent and remain elevated much longer (7-10 days) than amylase.
Elevations 2 to 50 times the upper reference have been reported. The increase in serum lipase is not necessarily proportional to the severity of the attack. Normalization is not necessarily a sign of resolution.
In acute pancreatitis, normoamylasemia may occur in up to 20% of such patients. Likewise, the existence of hyperlipemia may cause a spurious normoamylasemia. For these reasons, it is suggested that the 2 assays complement and not exclude each other, and that both enzymes should be assayed.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Patients should be fasting before the specimen is drawn.
Collection tubes with glycerol-lubricated stoppers or tubes containing citrate, oxalate, or EDTA should not be used.
Certain drugs such as cholinergics and opiates may elevate serum lipase.
Renal disease may elevate the serum lipase.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Tietz Textbook of Clinical Chemistry. Edited by CA Burtis and ER Ashwood. Philadelphia, WB Saunders Company, 2001
2. Swaroop VS, Chari ST, Clain JE: Acute pancreatitis. JAMA 2004;291:2865-2868
Method Description Describes how the test is performed and provides a method-specific reference
The lipase method is an enzymatic colorimetric method in which lipase catalyzes the hydrolysis of a natural 1,2-diglyceride to form monoglyceride and fatty acid. Monoglyceride is hydrolyzed by monoglyceride lipase to form glycerol and fatty acid. Glycerol is then phosphorylated by glycerol kinase in the presence of ATP to form glycerol-3-phosphate, which is oxidized by glycerol-3-phosphate oxidase to form dihydroxyacetone phosphate and hydrogen peroxide. Subsequently, hydrogen peroxide reacts with 4-aminoamtipyrine and sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase to form quinone diimine dye. The dye absorbs light at 550 nm. The rate of increase in absorbance at 550 nm is directly proportional to the pancreatic lipase activity in the specimen. The method is sensitive and specific for pancreatic lipase and utilizes co-lipase and deoxycholate as activators.(Package insert: Equal Diagnostics Lipase reagent, Exton, PA)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|