NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
The identification of Cyclospora as a cause of infectious gastroenteritis
See Parasitic Investigation of Stool Specimens Algorithm in Special Instructions for other diagnostic tests that may be of value in evaluating patients with diarrhea.
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
See Parasitic Investigation of Stool Specimens Algorithm in Special Instructions.
Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test
Safranin Stain of Stool
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Coccidian like bodies
Cyanobacter like Bodies
Coccidian like bodies
Cyanobacter like Bodies
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Patient should avoid use of anti-diarrheal medication (ie, loperamide or Pepto-Bismol).
The presence of barium will interfere with this test.
Submit only 1 of the following specimens:
Specimen Type: Preserved stool
Preferred: ECOFIX preservative (Supply T219)
Acceptable: Container with 10% formalin
Specimen Volume: 10 g
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Unpreserved stool
Container/Tube: Stool container (Supply T288)
Specimen Volume: 5 g
Collection Instructions: See Stool Collection Information Sheet in Special Instructions.
Specimen Stability Information: Refrigerated 3 days (preferred)/Frozen
Specimen Type: Duodenal aspirate
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Preserved stool: 1 g/Unpreserved stool: 2 g
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
In recent years, Cyclospora cayatenensis has been shown to cause (in humans) gastroenteritis characterized by watery diarrhea and systemic symptoms such as anorexia, malaise, weight loss, and general debilitation.
The organism which was originally thought to be a cyanobacteria or blue-green algae is now recognized as a protozoan parasite belonging to the coccidian group. It is similar in morphology and staining to Cryptosporidium but is approximately twice as large.
Diarrhea caused by Cyclospora has been reported in Nepal, the Indian subcontinent, Southeast Asia, and Latin America.
It has been extensively studied in Peru and the species name derives from the university where this work was done.
Although most cases of cyclosporiasis have been seen in travelers to developing countries, a focal outbreak due to contaminated water occurred at a Chicago medical center in 1990. Also, in the summer of 1996, a widespread outbreak occurred in many areas of the United States and Canada due to the importation of contaminated raspberries from Guatemala.
Transmission is probably fecal-oral and can be food borne or water-borne.
The infection is usually self-limited but symptoms can be prolonged. The infection usually responds to treatment with a sulfa-trimethoprim combination.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
If positive, reported as Cyclospora species
A report of Cyclospora species indicates the presence of this parasite in the patient's feces.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
The full spectrum of disease and the extent of carriage without disease have not been determined. In addition, the exact sensitivity and specificity of the stain have also not been determined.
Cryptosporidium also stain acid-fast although they are much smaller than Cyclospora. Cryptosporidium can be specifically identified using the antigen detection enzyme-linked immunosorbent assay (ELISA).
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Soave R: Cyclospora: an overview. Clin Infect Dis 1996;23:429-437
Method Description Describes how the test is performed and provides a method-specific reference
Safranin stain of stool concentrate. Fecal specimens may be submitted fresh or preserved in formalin or ECOFIX. Prepare a thin smear of the fecal material (preserved fecal specimens or fecal concentrate) to be stained. Air dry the smear. Place in acidic alcohol (3%) for 5 minutes. Wash slides with cold tap water and place slides in coplin jar containing safranin (1%) solution in acidified water (pH 6.5) and microwave at full power (650w) for 1 minute. Wash off excess stain with tap water. Place the slides in a coplin jar containing an aqueous solution of either 1% methylene blue or 1% malachite green and let stand for 1 minute. Rinse gently with tap water, let dry and mount with a number 1 coverslip. The oocysts of Cyclospora will stain a brilliant reddish orange.(Visvesvara GS, Moura H, Kovacs-Nace E, et al: Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J Clin Microbiol 1997;35:730-733)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 12 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|