Mumps Virus Antibody, IgM, Serum
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Laboratory diagnosis of mumps virus infection
Enzyme Immunoassay (EIA)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Mumps Ab, IgM, S
Soft ID: MUMPM
Soft ID: MUMPM
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 1 mL
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Mild OK; Gross reject
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Serum||Refrigerated (preferred)||14 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Mumps virus, together with parainfluenza types 1 through 4, respiratory syncytial virus, and measles virus are classified in the family Paramyxovirdae. Mumps is an acute infection which causes the painful enlargement of the salivary glands in approximately 70% to 90% of children (4-15 years of age) who develop clinical Disease.(2) In 5% to 20% of postpubertal individuals, testicular pain (orchitis in males) and abdominal pain (oophoritis in females) can occur. Other complications include pancreatitis (<5% of cases) and central nervous system disease (meningitis/encephalitis) that occur rarely (about 1 in 6,000 cases of mumps). Widespread routine immunization of infants with attenuated mumps virus has changed the epidemiology of this virus infection. Since 1989, there has been a steady decline in reported mumps cases, with and average of 265 cases each year since 2001. However, a recent outbreak of mumps in 2006 re-emphasized that this virus continued to persist in the population, and laboratory testing may be needed in clinically compatible situations.
The laboratory diagnosis of mumps is typically accomplished by detection of antibody to mumps virus. However, due to the limitations of serology (eg, inadequate sensitivity and specificity), additional laboratory testing including virus isolation or detection of viral nucleic acid by PCR in throat, saliva or urine specimens should be considered in clinically compatible situations.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative (reported as positive, equivocal, or negative)
Index value 0.00-0.79=negative
Positive: presence of IgM-class antibodies to mumps virus may support a clinical diagnosis of recent/acute phase infection with this virus.
Negative: absence of IgM-class antibodies to mumps virus suggests lack of acute phase infection with mumps virus. However, serology may be negative in early disease, and results should be interpreted in the context of clinical findings.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Results must always be interpreted together with other clinical and laboratory findings.
Serum specimens drawn during the acute phase of infection may be negative by serological tests.
All positive results must be interpreted with care, as some false-positive results or heterotypical responses of the IgM have been seen in the serum of pregnant women or in patients with an acute infection caused by cytomegalovirus, herpes simplex virus, measles, rubella, and parvovirus.
SeraQuest mumps IgM test kit showed a sensitivity of 97.3% and a specificity of 96.6% when 160 specimens were tested in parallel with a reference method. (Package insert: SeraQuest, 1938 NE 148th Terrace, North Miami FL)
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Harmsen T, Jongerius MC, van der Zwan CW, et al: Comparison of a neutralization enzyme immunoassay and an enzyme-linked immunosorbent assay for evaluation of immune status of children vaccinated for mumps. J Clin Microbiol 1992 Aug;30(8):2139-2144
2. Hodinka RL, Moshal KL: Childhood infections. In Essentials of Diagnostic Virology. Edited by GA Storch. Churchill Livingstone, New York. 2000, pp 168-178
Method Description Describes how the test is performed and provides a method-specific reference
The SeraQuest mumps IgM is an enzyme capture method. Diluted samples are incubated in wells coated with anti-human IgM monoclonal antibodies. If present, IgM antibodies are captured in the wells. Wells are washed, removing excess sample. Conjugate-antigen complex (mumps antigen in a complex with monoclonal antibodies conjugated to horseradish peroxidase) is added and wells are incubated. IgM antibodies specific for the antigen will bind the conjugate. Wells are washed, removing excess conjugate. Peroxidase substrate is added and wells are incubated. Stop solution is added converting the substrate to a yellow end product which is read photometrically.(Package insert: Mumps IgM, SeraQuest, 1938 NE 148th Terrace, North Miami, FL 33181)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 4 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|MUMP1||Mumps Ab, IgM, S||6478-2|