Test ID: BRUC
Brucella Culture
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Diagnosis of brucellosis
Method Name A short description of the method used to perform the test
Conventional Culture Technique
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen source is required.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Bone marrow
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Stability Information: Ambient
Alternate:
Specimen Type: Abscess, respiratory specimen, spinal fluid, sterile body fluid, or tissue
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Stability Information: Refrigerated
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Brucella are facultative intracellular gram negative staining bacilli capable of producing the disease "brucellosis" in humans. Human disease likely is acquired by contact with animals infected with the organism (Brucella abortus, Brucella suis, Brucella melitensis, and occasionally Brucella canis) either by direct contact or by ingestion of meat or milk. The signs and symptoms associated with brucellosis may include fever, night sweats, chills, weakness, malaise, headache, and anorexia. The physical examination may reveal lymphadenopathy and hepatosplenomegaly. A definitive diagnosis of brucellosis is made by recovering the organism from blood, fluid (including urine), or tissue specimens.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative (reported as positive or negative)
Interpretation Provides information to assist in interpretation of the test results
Isolation of a Brucella species indicates infection.
Cultures of blood and/or bone marrow are positive in 70% to 90% of acute Brucella infections, but much less so in subacute or chronic infections. In these latter instances, culture yield is highest from the specific tissue involved, or serology may be necessary to establish diagnosis
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Although brucellae are relatively resistant to adverse environmental conditions, excessive delay in the transport of material for culture to the laboratory may result in a lower yield.
Mayo Medical Laboratories does not perform species identification of Brucella; these isolates should be submitted to the State Health Department laboratory.
If a significant delay is expected, specimens should be refrigerated.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Mandell GL, Bennett JE, Dolin R: Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. Fourth edition. New York, Churchill Livingstone, 1995, pp 2053-2060
Method Description Describes how the test is performed and provides a method-specific reference
Specimens are inoculated onto buffered charcoal yeast extract agar (BCYE) or GC-Lect agar for urines and respiratory specimens and chocolate blood agar incubated at 35 degrees C with increased CO2. Cultures are incubated for 14 days before issuing a negative report. Conventional biochemicals are used for identification.(Shapiro DS, Wong JD: Brucella. In Manual of Clinical Microbiology. Seventh edition. Edited by PR Murray, EJ Baron, MA Pfaller, et al. Washington, DC, ASM Press, 1999, pp 625-631)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday, Wednesday, Friday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
87081-Brucella culture
87077-Gram negative Bacillus identification (if appropriate)
87077-Identification by MALDI-TOF mass spec (if appropriate)
87077-Additional identification procedure (if appropriate)
87153-Aerobe identification by sequencing (if appropriate)
87176-Tissue processing (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| BRUC | Brucella Culture | In Process |
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