NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Monitoring propafenone therapy
High-Performance Liquid Chromatography (HPLC)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Container/Tube: Red top
Specimen Volume: 3 mL
Collection Instructions: Draw blood after patient has been receiving propafenone for at least 3 days and just before administration of the next dose.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Mild OK; Gross reject
Mild OK; Gross reject
Plasma, serum gel tube
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Serum Red||Refrigerated (preferred)||14 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Tocainide, mexiletine, flecainide, and propafenone are all class I antiarrhythmic agents whose dominant cardiac effect is to reduce the rate of rise of the action potential in cardiac cells. In so doing, they increase the threshold of excitability of myocardial cells, depress the conduction velocity of the impulse around the heart, and prolong the defective refractory period, which results in stabilization of the heart rate. All 4 drugs are effective following oral administration.
Tocainide, mexiletine, and flecainide undergo minimal first-pass metabolism and have relatively long half-lives (10-16 hours). In contrast, propafenone undergoes extensive first-pass metabolism (half-life is approximately 1-3 hours). Its clinical efficacy is maintained through the formation of a metabolite (5-hydroxypropafenone) that is more pharmacologically active than the parent drug and has a longer plasma half-life (6-12 hours).
Propafenone is primarily used to treat ventricular arrhythmias (ventricular tachycardia, supraventricular tachycardia, and ventricular premature contractions).
Specimens should only be drawn after patient has been receiving propafenone orally for at least 3 days. Trough concentrations should be drawn just before administration of the next dose.
Adverse side effects are seen in the central nervous system, skin, and gastrointestinal tract.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Therapeutic concentration: 0.5-2.0 mcg/mL
The therapeutic concentration is 0.5 to 2.0 mcg/mL; concentrations <0.5 mcg/mL likely indicate inadequate therapy and propafenone >2.0 mcg/mL indicates excessive therapy.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Antman EM, Beamer AD, Cantillon C, et al: Long-term oral propafenone therapy for suppression of refractory symptomatic atrial fibrillation and atrial flutter. J Am Coll Cardiol 1988;12:1005-1011
Method Description Describes how the test is performed and provides a method-specific reference
Propafenone is extracted into N-butyl chloride from serum made basic by the addition of a borate buffer. The N-butyl chloride is transferred into a clean conical tip tube and dried under nitrogen. The residue is reconstituted in mobile phase and an aliquot is analyzed by HPLC. Sensitivity limit of the method is 0.1 mcg/mL.(Scott RE, Johnson P, Moyer TP: Simultaneous analysis of five new class I antiarrhythmic drugs. Clin Chem 1988;34:1251)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday, Wednesday, Friday; 9:00 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|