NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Detection of a nutritional deficiency of carotene
Detection of excessive ingestion of carotene
High-Performance Liquid Chromatography (HPLC)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Collection Container/Tube: Red top
Submission Container/Tube: Amber vial (Supply T192)
Specimen Volume: 1 mL
Collection Instructions: Send specimen in amber vial to protect from light.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross OK
Mild OK; Gross OK
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Serum||Refrigerated (preferred)||14 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Beta-carotene is a member of the family of carotenoids, highly pigmented (red, orange, yellow) fat-soluble vitamins that are the precursors or provitamins of vitamin A. The principle provitamin A compounds include beta-carotene, alpha-carotene, and beta-cryptoxanthin. Carotenoids occur in high levels in many fruits and vegetables such as carrots, sweet potatoes, cantaloupe, and others. The most significant effect of these provitamins is their conversion to vitamin A, which plays a major role in vision as well as reproduction, embryonic growth, and immune function.
The highest levels of carotene can be found in the serum of individuals ingesting large amounts of vegetables, primarily carrots. These people may have a slight yellowish tinge of the skin but the sclera of the eye is not discolored. Decreased serum levels may be seen in individuals with nutritional deficiencies including anorexia nervosa, malabsorption, and steatorrhea.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
> or =18 years: 2.3-65.0 mcg/dL
<18 years: not established
Low beta-carotene concentrations in the serum are indicative of vitamin insufficiency. Elevated levels of beta-carotene may have clinical implications.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Lower serum beta-carotene concentrations have been associated with smoking and ethanol consumption.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Institute of Medicine, Food and Nutrition Board. Dietary reference intakes for vitamin C, vitamin E, selenium, and carotenoids. Washington, DC: National Academy Press; 2000 pp 325-382
2. Tietz Textbook of Clinical Chemistry. Fourth edition. Edited by CA Burtis, ER Ashwood, DE Bruns. St. Louis, MO, Elsevier Saunders, 2006
3. Gueguen S, Herbeth B, Siest G, Leroy P. An isocratic liquid chromatographic method with diode-array detection for the simultaneous determination of u-tocopherol, retinol, and five carotenoids in human serum. J Chromatogr Sci 2002;40:69-76
4. Thibeault D, Su H, MacNamara E, Schipper H: Isocratic rapid liquid chromatographic method for simultaneous determination of carotenoids, retinol, and tocopherols in human serum. J Chromatogr B Analyt Technol Biomed Life Sci 2009;877:1077-1083
Method Description Describes how the test is performed and provides a method-specific reference
An analog of beta-carotene (trans-beta-apo8’-carotenal) is added to the patient sample as an internal standard. Protein is precipitated from the mixture by the addition of ethanol. Beta-carotene and internal standard are then extracted from the resulting mixture by a liquid/liquid extraction with hexane. Beta-carotene and internal standard are separated utilizing high performance liquid chromatography. Beta-carotene is monitored at a 450nm wavelength and identified and quantitated by comparison of retention times and peak area ratio from the unknown with those obtained following the analysis of extracted standards.(Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies, batched 2 times per week
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|