Riboflavin (Vitamin B2), Plasma
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Evaluation of persons who present the signs of ariboflavinosis
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Riboflavin (Vitamin B2), P
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Collection Container/Tube: Green top (heparin)
Submission Container/Tube: Amber vial (Supply T192)
Specimen Volume: 0.50 mL
1. Fasting-overnight (12-14 hours) (infants-draw prior to next feeding).
2. Send specimen in amber vial to protect from light.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
0.25 mL plasma
Mild OK; Gross OK
Mild OK; Gross reject
Mild OK; Gross OK
Plasma gel tube, EDTA, or serum
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Plasma Heparin||Frozen (preferred)||14 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
There are 3 principle vitamin B2-active flavins found in nature-riboflavin, riboflavin 5-phosphate (flavin mononucleotide [FMN]), and riboflavin-5'-adenosyl-diphosphate (flavin adenosine dinucleotide [FAD]). In biological tissues, FMN and FAD serve as prosthetic units for a large variety of flavoproteins, which are hydrogen carriers in oxidation-reduction processes.
Dietary deficiency of riboflavin (ariboflavinosis) is characterized by sore throat, cheilosis (lesions on the lips), angular stomatitis (lesions on the angles of the mouth), glossitis (fissured and magenta-colored tongue), corneal vascularization, dyssebacia (red, scaly, greasy patches on the nose, eyelids, scrotum, and labia), and normocytic, normochromic anemia. Severe riboflavin deficiency may affect the conversion of vitamin B6 to its coenzyme, as well as conversion of tryptophan to niacin.
Riboflavin has a low level of toxicity and no case of riboflavin toxicity in humans has been reported. The limited absorptivity of riboflavin and its ready excretion in the urine normally preclude a health problem due to increased intake of riboflavin.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Normal: 1-19 mcg/L
Low concentrations in the blood plasma are indicative of nutritional deficiency. Concentrations <1 mcg/L are considered significantly diminished. Marginally low levels probably represent nutritional deficiency that should be corrected.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Testing of nonfasting specimens or the use of dietary vitamin B2 supplementation can result in elevated plasma vitamin B2 concentrations.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Russell RM, Suter PM: Vitamin and trace mineral deficiency and excess. In Harrison's Principles of Internal Medicine. 17th edition. Edited by AS Fauci, DL Kasper, E Braunwald, et al. New York, McGraw-Hill Book Company, 2008, pp 441-449
2. Ball GFM: Vitamins: their role in the human body. Oxford, Blackwell Publishing, 2004, pp 289-299
3. McCormick DB: Riboflavin. In Modern Nutrition in Health and Disease. 10th edition. Edited by ME Shils, M Shike, AC Ross, et al. Philadelphia, Lippincott Williams and Wilkins, 2006, pp 434-441
4. Hustad S, McKinley MC, McNulty H, et al: Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation. Clin Chem 2002;48:1571-1577
5. Midttun O, Hustad S, Solheim E, et al: Multianalyte quantification of vitamin B6 and B2 species in the nanomolar range in human plasma by liquid chromatography-tandem mass spectrometry. Clin Chem 2005;51:1206-1216
6. Capo-chichi CD, Gueant JL, Lefebvre E, et al: Riboflavin and riboflavin-derived cofactors in adolescent girls with anorexia nervosa. Am J Clin Nutr 1999;69:672-678
Method Description Describes how the test is performed and provides a method-specific reference
The stable isotope riboflavin 13C15N2 is added to plasma as an internal standard. Meta-phosphoric acid solution is then added to precipitate the proteins. Following sedimentation of the proteins, an aliquot of the clarified supernatant fluid is subjected to separation of riboflavin and internal standard from other plasma components by reverse-phase HPLC with quantitation by tandem mass spectrometry (LC-MS/MS).(Unpublished Mayo methods)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
2 days (not reported on Saturday or Sunday)
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|61637||Riboflavin (Vitamin B2), P||2924-9|