Test ID: TCMPF
T- and B-Cell Quantitation and Lymphocyte Proliferation to Mitogens Panel
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Assessing global T-cell function and quantitating lymphocyte subsets in a single orderable test.
This panel is most helpful when evaluating patients with immunodeficiencies, where quantitative decreases in T cells can lead to reduced functional mitogen responses.
See individual test IDs for additional information.
Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TBBS | T- and B-Cell QN by Flow Cytometry | Yes | Yes |
| LPMGF | Lymphocyte Proliferation, Mitogens | Yes | Yes |
Method Name A short description of the method used to perform the test
Flow Cytometry
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
B Cell
B-Cell
Blastogenesis Mitogens
CD19 Count, Flow Cytometry
CD3 Count, Flow Cytometry
CD4 Count, Flow Cytometry
CD56 Count, Flow Cytometry
CD8 Count, Flow Cytometry
Flow Cytometry, T- and B- Cells
Helper Suppressor Ratio
Immune Competence
Immune Status, Flow Cytometry
Immunodeficiency Panel, Flow Cytometry
Immunophenotyping-CD4 Count, Flow Cytometry
In Vitro T-Cell Function
Lymphocyte Blastogenesis Mitogens
Lymphocyte Surface Marker Assay
Mitogen Studies
Mitogens
PHA Phytohemagglutinin
Phytohemagglutinin (PHA)
Pokeweed (PWM)
PWM (Pokeweed)
Quantitative CD4 and CD8
Suppressor Helper Ratio
T & B Lymphocyte Surface Marker
T & B-Cell Surface Markers: T-Helper/T-Suppressor, Flow Cytometry
T and B Cells
T Cell
T-Cell
T-Helper/T-Suppressor Ratio
T4/T8 Helper Suppressor Ratio
Specimen Type Describes the specimen type needed for testing
Whole Blood EDTA
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Send specimens Sunday through Thursday only. Specimens must arrive within 24 hours of draw and by 10 a.m. on Friday. Draw and package specimens as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (Supply T668).
Date and time of draw and ordering physician's name and phone number are required.
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Two separate specimens are required.
Specimen Type: EDTA whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Type: Sodium heparin whole blood
Container/Tube: Green top (sodium heparin)
Specimen Volume:
<3 months: 1 mL
3 months-5 years: 2 mL
6-18 years: 3 mL
>18 years: 10 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross reject |
| Icterus | NA |
| Other | Specimens in aliquot tubes (not in original Vacutainer) |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours |
| Whole Blood EDTA | Ambient | 48 hours |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
See individual test IDs.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
The appropriate age-related reference values will be provided on the report.
Interpretation Provides information to assist in interpretation of the test results
Since the presence of lymphopenia, particularly in the T-cell compartment, can reduce the magnitude of the proliferative response to antigens, it is helpful to perform simultaneous numerical evaluation of lymphocyte subsets to aid in interpretation of the functional (proliferation) data.
See individual test IDs for additional information.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
See individual test IDs.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
See individual test IDs.
Method Description Describes how the test is performed and provides a method-specific reference
See individual test IDs.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
T- and B-Cell quantitation by flow cytometry: Monday through Sunday
Lymphocyte proliferation to mitogens: Monday through Friday
Do not send specimen after Thursday.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
T- and B-Cell Quantitation by Flow Cytometry
86355-B cells, total count
86357-Natural killer (NK) cells, total count
86359-T cells, total count
86360-Absolute CD4/CD8 count with ratio
Lymphocyte Proliferation to Mitogens, Blood
86353
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| 32317 | Interpretation | 69052-9 |
| 9336 | T-and B-Cell QN by Flow Cytometry(TBBS_) | In Process |
| 3321 | CD45 Lymph Count, Flow | 27071-0 |
| 32318 | Viab of Lymphs at Day 0 | In Process |
| 32321 | Max Prolif of PWM as % CD45 | In Process |
| 3316 | % CD3 (T Cells) | 8124-0 |
| 3318 | % CD19 (B Cells) | 8117-4 |
| 32322 | Max Prolif of PWM as % CD3 | In Process |
| 32323 | Max Prolif of PWM as % CD19 | In Process |
| 4054 | % CD16+CD56 (NK cells) | 18267-5 |
| 3319 | % CD4 (Helper Cells) | 8128-1 |
| 32319 | Max Prolif of PHA as % CD45 | In Process |
| 32320 | Max Prolif of PHA as % CD3 | 57741-1 |
| 3320 | % CD8 (Supp'r Cells) | 8138-0 |
| 32324 | Mitogen Comment | 48767-8 |
| 3322 | CD3 (T Cells) | 8122-4 |
| 3324 | CD19 (B Cells) | 8116-6 |
| 4055 | CD16+CD56 (NK cells) | 20402-4 |
| 3325 | CD4 (Helper Cells) | 8127-3 |
| 3326 | CD8 (Supp'r Cells) | 8137-2 |
| 3327 | H/S Ratio | 8129-9 |
| 6657 | Comment | 48767-8 |
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