Test ID: EXHBM
Hematologic Disorders, DNA/RNA Extract and Hold, Bone Marrow
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Reserving nucleic acid on any specimen for which molecular analysis may be necessary at a future date, ensuring that adequate material for testing is available
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
DNA and total RNA will be extracted from the specimen and stored at -80 degrees C for 1 year from the date the extraction process was performed. A report of "Performed" will be sent and a $75.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratories test menu, call 800-533-1710. Additional charges will be assessed for testing performed.
Method Name A short description of the method used to perform the test
Nucleic Acid Extraction
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
DNA and RNA Extract and Hold
DNA Extract and Hold
RNA Extract and Hold
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen must arrive within 72 hours of draw.
Collection Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: ACD
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send specimen in original tube.
Additional Information:
1. Date of draw is required.
2. To request molecular testing from the Molecular Hematopathology Laboratory, call 800-533-1710. Additional charges will be assessed for testing performed.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | NA |
| Icterus | NA |
| Other | Paraffin-embedded material |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Bone Marrow | Ambient (preferred) | 72 hours |
| Refrigerated | 72 hours |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
It is frequently useful to obtain nucleic acid from clinical specimens containing a hematopoietic neoplasm at the time of diagnosis, so that appropriate material is available for molecular analysis should subsequent testing be required. For example, when a diagnosis of acute myelogenous leukemia is made, there is a delay before karyotype information, which determines whether testing for molecular prognostic markers is necessary, is available. After this delay, the diagnostic sample is usually no longer available or the nucleic acid has degraded to such an extent that it is no longer adequate for testing. Thus, it is useful to obtain nucleic acid on such specimens promptly at diagnosis and retain it until it is known whether additional testing is necessary.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Not applicable
Interpretation Provides information to assist in interpretation of the test results
A report of "Performed" will be sent and a $75 processing fee will be assessed. No interpretation will be given. Should the sample be used in future testing, interpretation would be incorporated with the final testing.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
The nucleic acid will be held at -80 degrees C for 1 year from the time of extraction. The nucleic acid may only be used for suitable tests offered by the Molecular Hematopathology Laboratory at Mayo Clinic. Nucleic acid cannot be shipped to another institution for testing and cannot be released for research use.
Only bone marrow will be processed. Paraffin-embedded material and fresh, solid tissue will not be routinely accepted at this time.
Method Description Describes how the test is performed and provides a method-specific reference
DNA is extracted using an automated system (Qiagen EZ1 BioRobots). RNA is extracted using a commercial kit (Qiagen miRNeasy). Both systems include a sample lysis step followed by binding of nucleic acid to a solid phase for separation from other cellular components and a final nucleic acid elution step.(Package insert: miRNeasy Mini Handbook. Qiagen, Oct 2007)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday
Specimens received Saturday or Sunday will be processed on Monday.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| 60558 | DNA and RNA Extraction | N/A |
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