Test ID: FBORE
Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FBAGI | B. pertussis Ab, IgG Immunoblot | No | No |
| FBAMI | B. pertussis Ab, IgM Immunoblot | No | No |
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
If Bordetella pertussis Antibody, IgG by ELISA is 2.5 U/mL or greater, then B. pertussis Ab, IgG Immunoblot testing will be performed at an additional charge.
If Bordetella pertussis Antibody IgM by ELISA is 1.2 U/mL or greater, then B. pertussis Ab, IgM Immunoblot testing will be performed at an additional charge.
Method Name A short description of the method used to perform the test
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/
Qualitative Immunoblot
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Draw blood in a plain, red-top tube(s). (Serum gel tube is acceptable.) Separate from cells within 2 hours of collection. Send 1 mL of serum refrigerate in a plastic vial.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis: | NA |
| Thawing: | Warm reject; Cold OK |
| Lipemia: | Mild OK; Gross OK |
| Icterus: | NA |
| Other: | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 365 days | |
| Ambient | 48 hours |
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
B. pertussis Ab, IgG by ELISA <=2.4 U/mL
B. pertussis Ab, IgM by ELISA <=1.1 U/mL
Recommend that treatment decisions be based on the result of the B.pertussis IgM immunoblot instead of the ELISA test. B.pertussis IgM test by ELISA may produce false-positive results.
Test Performed By: ARUP Laboratories
500 Chipeta Way
Salt Lake City, UT 84108
Interpretation Provides information to assist in interpretation of the test results
Detectable levels of IgG antibodies to B. pertussis may be
seen in the serum of vaccinated individuals of all ages, in
early infancy as the result of placental transfer, and in
the convalescent phase of a recent infection. IgG antibodies
can only be used to diagnose active infection when paired
sera are available and a rise in antibody level can be
demonstrated. A significant rise may not always be demon-
strated as peak levels of IgG may be reached before the
first sample is collected. Therefore, IgA and IgM antibody
levels should be measured to diagnose active disease.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Tuesday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
86615/x2
86615 (per immunoblot if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| Z2614 | B. pertussis Ab, IgG | In Process |
| Z2615 | B. pertussis Ab, IgM | In Process |
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