FFES/91215 Overview: Estradiol Free, Serum (includes Estradiol and SHBG)

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Estradiol Free, Serum (includes Estradiol and SHBG)

Method Name A short description of the method used to perform the test

Mass Spectrometry

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

Free Estradiol

Aliases Lists additional common names for a test, as an aid in searching

Estradiol, Free, Serum FORWARD
Free Estradiol, Serum FORWARD

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Draw blood in a plain, red-top tube or serum gel tube. Spin down, pour off into plastic vial within 1 hour of collection, and send 3 mL serum frozen.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

1.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Specimens other than


Anticoagulants other than










Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
SerumFrozen (preferred)180 days
 Ambient 5 days
 Refrigerated 5 days

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Free Estradiol, Percent

Reference Ranges (%)

      Age                        Range

Adult Males                   1.7 - 5.4

Adult Females               1.6 - 3.6


Free Estradiol, Serum

Reference Ranges (pg/mL)

      Age                        Range

Adult Males                   0.2 - 1.5

Adult Females               0.6 - 7.1


Sex Hormone Binding Globulin (SHBG), Serum

Reference Ranges (nmol/L)

      Age                                    Range

 1 - 23m                                    60 - 252

Prepubertal (24m - 8y)                72 - 220


  Males                                     16 - 100

  Females                                  36 - 125

Adult Males                               20 - 60

Adult Females

   Premenopausal                       40 - 120

   Postmenopausal                     28 - 112


Estradiol, Serum

Reference Ranges (pg/mL)


       Age                       Range

Newborn                        Levels are markedly elevated at birth and fall rapidly during the first week to prepubertal values of <15

Males <6m                    Levels increase to 10 - 32 between 30 and 60 days, hen decline to prepubertal levels of <15 by six months.

Females <1y                 Levels increase to 5.0 - 50 between 30 and 60 days, then decline to prepubertal levels of <15 during the first year.

Prepubertal                    <15

Adult Males                   8.0 - 35

Adult Females

 Follicular                      30 - 100

 Luteal                          70 - 300

 Postmenopausal           <15



Test Performed By:   Esoterix Endocrinology

                                        4301 Lost Hills Road

                                        Calabasas Hills, CA 91301

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.


Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7 - 10 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

9 - 12 days

Performing Laboratory Location The location of the laboratory that performs the test

Esoterix Endocrinology

Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.

ASR - Analyte Specific Reagent for Sex Hormone Binding Globulin (SHBG), Serum This test was developed and its performance characteristics determined by Esoterix. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance is not necessary. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is regulated under the Clinical Laboratory Improvement Amendment (CLIA) of 1988 as qualified to perform high complexity clinical testing.

CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.


84270/Sex Hormone binding globulin

84999/Unlisted chemistry procedure

LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and result codes of this test.

LOINC codes are provided by the performing laboratory.

Test IDTest Order NameOrder LOINC Value
FFESFree EstradiolIn Process


Result IDTest Result NameResult LOINC Value
Z0228Free Estradiol, Percent14960-9
Z0229Free Estradiol, Serum2240-0
Z0230Sex Hormone Binding Globulin (SHBG)13967-5
Z0259Estradiol, Serum35384-7