AFPA - Specimen: Alpha-Fetoprotein, Amniotic Fluid

Test Catalog

Test Name

Test ID: AFPA    
Alpha-Fetoprotein, Amniotic Fluid

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

If alpha-fetoprotein (AFP) is positive, then acetylcholinesterase (AChE) will be performed at an additional charge.

Specimen Type Describes the specimen type needed for testing

Amniotic Fld

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Amniotic fluid container

Specimen Volume: 1 mL

Collection Instructions: Do not centrifuge.

Additional Information:   

1. The following information is required:

a. Date ultrasound performed

b. Estimated due date by ultrasound

c. Collection date

d. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.

2. If chromosome studies are also requested, see AF / Chromosome Analysis, Amniotic Fluid. The specimen for AFP-AF testing, when requested with chromosome analysis, cannot be frozen.

Forms: Second Trimester Maternal Screening Alpha-Fetoprotein (AFP)/QUAD Screen Patient Information (T595) is required in Special Instructions.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected









Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Amniotic FldRefrigerated7 days