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Test ID: FH1N1    
Influenza A H1N1 (2009) Real-Time RT-PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Nasal, nasopharyngeal or throat swab, or nasal aspirates in 3 mL of viral transport media, M4 media or V-C-M medium (green cap) tube or equivalent (UTM) shipped refrigerated.

Note: 1. Swab must be sterile Dacron, nylon, or rayon with plastic shafts. Place swab in sterile viral transport media containing protein stabilizer, antibiotics to inhibit bacterial fungal growth, and buffer solution (eg, UTM, VCM, M4, M5, M6 and other media intended to transport Chlamydia, Mycoplasma or viruses).

2. Do not use calcium alginate swabs; only sterile swabs are acceptable.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.35 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Specimens other than

Nasal, nasopharyngeal swab, nasal aspirate

Anticoagulants other than

NA

Hemolysis

NA

Thawing

NA

Lipemia

NA

Icteric

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Frozen 30 days
 Ambient 48 hours