Test ID: FNTSM
Neurotransmitter Profile 3

Medical Neurogenetics collection kit (MML Supply T657) required.

Each collection kit contains 5 microcentrifuge tubes.

COLLECTION PROTOCOL

   1). CSF should be collected from the first drop into the tubes in the

         numbered order. Fill each tube to the marked line with the required

         volumes.

         Tube 1:  0.5 mL

         Tube 2:  0.5 mL

         Tube 3:  1.0 mL (contains antioxidants necessary to protect the

                                          sample integrity)

          Tube 4:  1.0 mL

          Tube 5:  0.5 mL

-          If samples not blood contaminated should be placed on dry ice

at bedside

-          If samples are blood contaminated, the tubes should immediately

be centrifuged (prior to freezing) and the clear CSF transferred to

new similarly labeled tubes, then frozen

-          Store samples at -80 until they can be shipped.

     2). Complete Medical Neurogenetics, LLC request form, marking the

            following three tests: Neurotransmitter Metabolites, Tetrahydro-

           biopterin and 5-Methyltetrahydrofolate. Also include sample date,

           date of birth, current medications and relevant history.

    3). Label tubes with patient name and ID number, leaving the tube number

          viewable.

    4). Place samples inside a specimen transport bag and the Medical

          Neurogenetics, LLC request form inside the pouch of the transport bag.

    5). Ship samples frozen on dry ice.