Unit Code 9070:
Coagulation Factor VIII Activity Assay, Plasma
Specimen Required
Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
See "Coagulation Studies" in Special Instructions.
1. Draw blood in a light blue-top (citrate) tube(s). Spin down, remove
plasma, spin plasma again, and place 1 mL of platelet-poor
plasma in plastic vial. (Glass vial is not acceptable.)
Note: Double-centrifuged specimen is critical for
accurate results as platelet contamination may
cause spurious results.
2. Freeze specimen immediately at < or = -40 degrees C, if possible.
3. Send specimen frozen.
Note: Each coagulation assay requested should have its
own vial.
4. If priority specimen, mark requisition, give reason, and request a
call-back.
Note: If ordering electronically, no form is required with the specimen.
If not ordering electronically, please complete and submit a
"Coagulation Request Form" (Supply T237) with the specimen.
Minimum Volume
Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
0.5 mL
The amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Transport Temperature
Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) and unacceptable transport temperature(s) are also included. The preferred transport temperature is listed first, followed by the alternate acceptable temperature (if appropriate) and lastly, the unacceptable transport temperature(s).
Frozen\Refrig NO\Ambient NO
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Specimens other than | Plasma |
| Anticoagulants other than | Sodium citrate |
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross reject |
| Icteric | NA |


