Test ID: BTKFP
Bruton's Tyrosine Kinase (BTK) Genotype and Protein Analysis, Full Gene Sequence and Flow Cytometry
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms:
1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions.
2. Bruton's Tyrosine Kinase (BTK) Genotype Patient Information Sheet (Supply T620) in Special Instructions
Ordering physician name and phone number are required.
For flow cytometry serial monitoring, we recommend that specimen draws be performed at the same time of day.
Specimens must arrive within 72 hours of draw. Send specimen Sunday through Thursday only. Draw and package specimen as close to shipping time as possible. Ship specimen overnight.
Two separate EDTA specimens are required:
Specimen Type: Blood for BTKSP/29305 Bruton's Tyrosine Kinase (BTK) Genotype, Full Gene Sequence and BTKSQ/89827 Bruton's Tyrosine Kinase (BTK) Genotype, Full Gene Sequencing
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label as BTKSP/29305 and BTKSQ/89827.
Additional Information:
1. Bone marrow transplants will interfere with testing.
2. Transfusions will interfere with testing for up to 4 to 6 weeks. DNA obtained from white cells may not provide useful information for patients who received a recent transfusion of blood that was not leukocyte-reduced. Wait 4 to 6 weeks until transfused cells have left the patient's circulation before drawing the patient's blood specimen for genotype testing.
Specimen Type: Blood for BTK/89011 Bruton's Tyrosine Kinase (Btk), Protein Expression, Flow Cytometry, Blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 5 mL
Collection Instructions:
1. Send specimen in original tube. Do not aliquot.
2. Label as BTK/89011.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross reject |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 72 hours |
| Ambient | 72 hours |
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