CD20 on B Cells
Specimen Type Describes the specimen type needed for testing
Whole Blood EDTA
This is the correct test to order if specifically confirming the absence of B cells due to suspected primary humoral or combined immunodeficiency or evaluating for CD19 deficiency.
If desirous of only quantitatively measuring total CD19 or CD20+ B cells, order TBBS / T- and B-Cell Quantitation by Flow Cytometry or CD20B / CD20 on B Cells, respectively. Do not order the detailed analysis of B cell subsets for this purpose.
This test should not be ordered for a comprehensive evaluation of peripheral B-cell subsets. For evaluation of memory B-cell subsets, transitional B cells, mature and immature B cells, order IABCS / B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood.
This test should not be used for evaluating presence of CD20 on malignant or nonmalignant B cells. The following test should be used instead, TAE / Therapeutic Antibody by Flow Cytometry, which provides percent of B cells expressing CD19 or CD20 but does not provide absolute cell counts (cells/mcL).
Draw and package specimens as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw.
Date of draw is required.
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
1. Secondary aliquot tubes will be rejected.
2. Testing will be canceled if the specimen is not received ambient.
3. For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Mild OK; Gross reject
Secondary aliquot tubes
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Whole Blood EDTA||Ambient||4 days|