LEGRP - Specimen: Legionella species, Molecular Detection, PCR

Test Catalog

Test ID: LEGRP    
Legionella species, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Legionella DNA is unlikely.

 

Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Neurology Test Request Form (T732) (http://www.mayomedicallaboratories.com/it-mmfiles/neurology-request-form.pdf)

 

Specimen source is required.

 

Specimen Type: Respiratory

Sources: Sputum, tracheal secretions/aspirates, transtracheal aspirate, bronchial washing/aspirate, bronchoalveolar lavage, lung fluid or pleural fluid

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Fresh tissue or biopsy

Sources: Lung tissue

Container/Tube: Sterile container

Specimen Volume: Entire collection

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Fluid: 0.5 mL/Tissue: 5 mm(3)

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Tissue in formalin, formaldehyde, or acetone

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Frozen 7 days