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Test ID: 89213    
Estrogen/Progesterone Receptor, Semi-Quantitative Immunohistochemistry, Manual

Available on the App Store

Specimen Type Describes the specimen type needed for testing

Mixed

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

If being ordered for diagnostic purposes:

Order 70012 / Surgical Pathology Consultation and then request the stains.

 

If being ordered for prognostic purposes:

Specimen Type: Breast carcinoma

Preferred: A paraffin-embedded tissue block containing in-situ, invasive or metastatic breast carcinoma tissue that has been fixed in 10% neutral buffered formalin within 1 hour from surgical collection time and for a total of 6 to 72 hours and shipped at ambient temperature

Acceptable: 3 unstained sections, containing carcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature

 

Specimen Type: Non-breast carcinoma

Preferred: A paraffin-embedded tissue block containing carcinoma tissue that has been fixed in 10% neutral buffered formalin and shipped at ambient temperature

Acceptable: 3 unstained sections, containing carcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature

Container/Tube: Pathology Packaging Kit (Supply T554)

Collection Instructions:

1. Submit paraffin-embedded carcinoma tissue.

2. Include accompanying pathology report stating the final diagnosis. If not available, a preliminary diagnosis is acceptable.

3. Attach the green pathology address label included in the kit to the outside of the transport container.

Additional Information:

1. Information regarding fixative used, time to fixation and duration of fixation is required. When ordering 89213 / Estrogen/Progesterone Receptor, Semi-Quantitative Immunohistochemistry, Manual, the following questions, as stated on the order form or presented electronically, must be answered:

-"Was specimen fixed in 10% neutral buffered formalin within 1 hour from surgical collection time? Yes, No, or Unknown."

-"Has specimen been fixed in 10% neutral-buffered formalin for 6 to 72 hours? Yes, No, or Unknown."

2. According to the College of American Pathologists (CAP)/American Society of Clinical Oncology (ASCO) guidelines, estrogen/progesterone receptor protein immunohistochemical test results are only valid for nondecalcified, paraffin-embedded specimens fixed in 10% neutral buffered formalin within 1 hour from surgical collection time and for a total time of 6 to 72 hours. Delay to fixation, under- or overfixation may affect these results.

3. Paraffin blocks will be returned with final report.

4. Estrogen/progesterone receptor testing is not appropriate and not performed for cases of lobular carcinoma in situ.

Forms: If not ordering electronically, submit a Pathology/Cytology Request Form (Supply T246) with the specimen.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

NA

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
MixedAmbient (preferred)
 Frozen 
 Refrigerated