JAK2 Exon 12 and Other Non-V617F Mutation Detection, Blood
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
This is a second-order test that should be used when the test for the JAK2B / JAK2 V617F Mutation Detection, Blood test is negative. The sensitivity of this assay is much less than that of the JAK2B blood test. This is because the sequencing technique is required to evaluate for many potential mutations. The sensitive JAK2B blood test should always be performed first, as the JAK2 mutation burden may be very low in some specimens. If JAK2B blood test is negative, then this assay (JAKXB / JAK2 Exon 12 and Other Non-V617F Mutation Detection, Blood) should be performed for detection of non-V617F JAK2 mutations.
The following algorithms are available in Special Instructions:
-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
-Erythrocytosis Evaluation Testing Algorithm
Specimen Type Describes the specimen type needed for testing
Additional Testing Requirements
The sensitive JAK2B / JAK2 V617F Mutation Detection, Blood should always be performed first, as the JAK2 mutation burden may be very low in some specimens.
Specimen must arrive within 5 days (120 hours) of collection. Draw and package specimen as close to shipping time as possible.
Date of collection is required.
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Preferred: EDTA (lavender top)
Acceptable: ACD (yellow top)
Specimen Volume: 4 mL
1. Invert several times to mix blood.
2. Send specimen in original tube.
1. Hematopathology Patient Information (T676) in Special Instructions
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf).
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Moderately to severely clotted
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Whole blood||Refrigerated (preferred)||5 days|