|Values are valid only on day of printing.|
All routine viral cultures are inoculated into cell culture tubes for viral detection. The most common specimens received for routine testing include bronchoalveolar lavage, sputum, and throat. A rapid (16-hour incubation) shell vial cell culture assay will be inoculated when specimens are designated for herpes simplex virus or cytomegalovirus detection or as appropriate for source indicated.
-For oral specimens for suspected hand-foot-and-mouth disease or enterovirus, clearly indicate "enterovirus" on request.
Testing for Mumps or Measles Virus: Do not submit specimens for viral culture testing if there is a suspicion of mumps or measles virus. Suspect mumps or measles cases should be submitted directly to a state health department for laboratory testing.
Do not submit specimens for viral culture testing if there is a suspicion of Ebola virus or any viral hemorrhagic fever, avian influenza, severe acute respiratory syndrome (SARS), Middle Eastern respiratory syndrome Coronovirus (MERS-CoV), or other high-risk infectious agents. Contact your state health department for more information and testing options.
Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).
Specimen source is required. Specimen must be transported at refrigerate temperature.
Specimen Type: Lower respiratory tract specimens such as bronchoalveolar lavage, bronchial washings or aspirates, tracheal secretions or sputum
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Throat
Preferred: BBL CultureSwab (T092)
Acceptable: Dacron-tipped swab with plastic handle (T507)
Specimen Volume: Swab
Collection Instructions: Return swab to swab cylinder or multi-microbe medium (M5) (T484).
Specimen Type: Tissue
Sources: Lung and others
Container/Tube: Sterile container with 1-2 mL of sterile saline or M5 (T484)
Specimen Volume: Entire collection
Specimen Type: Oral
Container/Tube: Dacron-tipped swab with plastic handle (T507)
Specimen Volume: Swab
1. Place swab in M5 viral transport media.
2. Indicate "Looking for enterovirus" or "Looking for mumps" if applicable.
Frozen, gel swab, swab with wooden handle, lymph node swab or tissue, bone marrow or bone tissue, wound swab, or tissue. Swab with a wood handle has been shown to be toxic to some viruses and is not acceptable for culture. Gel swab is not acceptable for culture.