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Test ID: MTBRP    
Mycobacterium tuberculosis Complex, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycobacterium tuberculosis DNA is not likely.

                         

Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.

 

Preferred Specimens: Body fluid, cerebrospinal fluid (CSF), respiratory (eg, bronchoalveolar lavage fluid: BAL, bronchial washing, sputum), stool, tissue (fresh or paraffin),bone, bone marrow, or urine

 

Acceptable Specimens: If no fresh specimen is available, digested respiratory specimens treated with N-acetyl-L-cysteine (NALC)/NaOH are acceptable (eg, BAL, bronchial washing, respiratory fluid, sputum, or tracheal secretion), as are NALC/NaOH-treated gastric washings.

                            

Unacceptable Specimens: Blood, specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth), swab, tissue in formalin fluid, specimen >7 days old (with the exception of paraffin tissue)

 

Specimen must arrive within 7 days of collection; specimen >7 days will be rejected.

 

Specimen source is required.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Body fluid

Sources: Body, bone marrow aspirate, or CSF

Container/Tube: Sterile container

Specimen Volume: 1 mL

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Additional Information: Only fresh, non-NALC/NaOH-digested body fluid is acceptable.

 

Specimen Type: Gastric washing

Container/Tube: Sterile container

Specimen Volume: 10 mL

Collection Instructions: Neutralize specimen within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of gastric washing.

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

 

Specimen Type: Respiratory

Sources: BAL, bronchial washing, or sputum

Container/Tube: Sterile container

Specimen Volume: 3 mL

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

 

Specimen Type: Stool

Container/Tube: Sterile container

Specimen Volume: 5-10 g

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Additional Information: Only fresh, non-NALC/NaOH-digested stool is acceptable.

 

Specimen Type: Tissue

Sources: Fresh, paraffin, bone, bone marrow biopsy

Container/Tube:

Preferred: Sterile container

Acceptable: Biopsy specimen of tissue fixed with formalin in a paraffin block

Specimen Volume: 5-10 mm

Collection Instructions: Block must be sent for sectioning.

Specimen Stability Information:

Fresh tissue: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Fixed tissue (paraffin): Ambient

Additional Information: Only fresh, non-NALC/NaOH-digested tissue is acceptable.

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions: Collect a random urine specimen.

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Additional Information: Only fresh, non-NALC/NaOH-digested urine is acceptable.

                                

Acceptable:

NALC/NaOH-digested specimen must arrive within 7 days of digestion.

                                                               

Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, gastric washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

1. Submit digested specimen treated with NALC/NaOH.

2. Clearly indicate on container and order form that specimen is a digested specimen.

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Additional Information: If a single specimen is being shared between mycobacteria culture, acid-fast smear, and/or Mycobacterium tuberculosis PCR, a minimum volume of 1.5 mL for body fluid, 3 mL for respiratory specimen, or a pea-sized piece of tissue should be obtained. Specimen volumes less than indicated may decrease sensitivity of testing. If insufficient volume is submitted, test or tests will be canceled.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Body Fluid: 0.5 mL/Bone: 5 mm/Gastric Washing: 5 mL/Respiratory Specimen-Non-Digested: 1.5 mL/Stool: 5 g/Tissue: 5 mm/Urine: 0.5 mL/NALC-NaOH-digested Specimen: 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Blood, specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth), swab, tissue in formalin fluid, specimen >7 days old (with the exception of paraffin tissue)

 

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesVaries