Test ID: PHIV
HIV-1 Proviral DNA Qualitative Detection by PCR, Blood
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions:
1. Refrigerate EDTA-whole blood specimen within 6 hours of collection, and ship on ice pack in <24 hours.
2. If shipment will be delayed for >24 hours, freeze EDTA-whole blood specimen at -70 degrees C (up to 35 days) until shipment on dry ice.
Additional Information: This test is intended for diagnosis of acute HIV-1 infection, including for detection of HIV-1 infection in children <18 months of age when serologic tests are not useful (due to presence of maternal HIV antibodies).
Forms: If not ordering electronically, submit a General Request Form (Supply T239) with the specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross OK |
| Lipemia | Mild OK; Gross OK |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Frozen (preferred) | |
| Refrigerated | 72 hours | |
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