Test ID: TREC
T-Cell Receptor Excision Circles (TREC) Analysis, Blood
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
This assay is useful for longitudinal assessment of thymic function and naive T-cell production. For comprehensive thymic function analysis on pediatric patients or posthematopoietic cell transplantation, order this test and CD4RT/89504 CD4 T-Cell Recent Thymic Emigrants (RTE).
Send specimen Monday through Thursday only. Specimen should arrive within 48 hours of draw. Draw and package specimen under strict ambient conditions as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (Supply T668).
Container/Tube: Lavender top (EDTA)
Specimen Volume:
Adults: 10 mL
Pediatrics:
-Preferred volume for >1 year = 5 mL
-Preferred volume for < or =1 year old = 3 mL
Collection Instructions:
1. Do not draw specimen through a butterfly needle.
2. Send specimen in original tube.
Additional Information:
1. Ordering physician's name and phone number are required.
2. For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Forms: TREC Assay Patient Information Sheet (Supply T589) in Special Instructions
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross reject |
| Icterus | NA |
| Other | Specimen that does not arrive at ambient (20-25 degree C) temperature |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Ambient | 48 hours |
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