VIRNR - Specimen: Viral Culture, Non-Respiratory

Test Catalog

Test ID: VIRNR    
Viral Culture, Non-Respiratory

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

All routine viral cultures are inoculated into cell culture tubes for viral detection. The most common specimens received for routine testing include body fluid, rectal, spinal fluid, and stool. A rapid (16-hour incubation) shell vial cell culture assay will be inoculated when specimens are designated for herpes simplex virus (HSV) or cytomegalovirus (CMV) detection, or as appropriate for source indicated.

 

Blood, lymph node tissue, and bone marrow/bone tissue specimens are not good sources for viral culture and are frequently toxic to cell culture lines. Most molecular methods are appropriate for these specimen types (exception: bone tissue). See individual real-time PCR assay test descriptions.

 

Genital, synovial fluid, wound swab or tissue (includes pus, drainage, or abscess fluid) specimens are not acceptable for viral culture.

 

Ocular fluids (vitreous and aqueous) are generally of inadequate volume for viral cell culture and molecular testing is typically recommended for these samples. See individual real-time PCR assay test descriptions.

 

Oral specimens submitted for culture should be ordered as VRESP / Viral Culture, Respiratory.

 

Dermal specimens are only acceptable for enterovirus culture:

-For dermal specimens for hand-foot-and-mouth disease or enterovirus, clearly indicate "enterovirus" on request.

 

For requests to rule-out HSV infection in neonates (<1 month) on dermal specimens, order VHSV / Herpes Simplex Virus (HSV), Culture From Neonates.

 

Testing for Mumps or Measles Virus: Do not submit specimens for viral culture testing if there is a suspicion of mumps or measles virus. Suspect mumps or measles cases should be submitted directly to a state health department for laboratory testing.

 

Do not submit specimens for viral culture testing if there is a suspicion of Ebola virus or any viral hemorrhagic fever, avian influenza, severe acute respiratory syndrome (SARS), Middle Eastern Respiratory Syndrome Coronovirus (MERS-CoV), or other high-risk infectious agents. Contact your state health department for more information and testing options.

 

For requests for Cytomegalovirus (CMV) on bone marrow or urine specimen, see:

-LCMV / Cytomegalovirus (CMV), Molecular Detection, PCR

 

For requests for herpes simplex virus (HSV) or varicella-zoster virus (VZV) on genital or dermal specimen, see:

-LHSV / Herpes Simplex Virus (HSV), Molecular Detection, PCR

-LVZV / Varicella-Zoster Virus, Molecular Detection, PCR

-LHSVZ / Herpes Simplex Virus (HSV) and Varicella-Zoster Virus (VZV), Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Forms: If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

 

Specimen source is required. Specimen must be transported at refrigerate temperature.

 

Specimen Type: Body fluid

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Rectal

Container/Tube: Dacron-tipped swab with plastic handle (Supply T507)

Specimen Volume: Swab

Collection Instructions: Place swab in M5 viral transport media.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

 

Specimen Type: Stool

Container/Tube: Sterile container

Specimen Volume: 5-10 g

 

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, etc.

Container/Tube: Sterile container with 1-2 mL of sterile saline or multi-microbe medium (M5) (Supply T484)

Specimen Volume: Entire collection

 

Specimen Type: Dermal (for enterovirus only)

Container/Tube: Dacron-tipped swab with plastic handle (Supply T507)

Specimen Volume: Swab

Collection Instructions:

1. Place swab in M5 viral transport media.

2. Clearly label "enterovirus" to ensure proper handling and test setup.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Body Fluid or Spinal Fluid: 1 mL; Stool: 5 g; Urine: 0.5 mL; Tissue Biopsy: 5 mm

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Frozen, gel swab, swab with wood handle, blood, bone marrow/bone tissue, lymph node, dermal, genital, wound swab or tissue, pus, drainage, abscess or urine. Swab with a wood handle has been shown to be toxic to some viruses and is not acceptable for culture. Gel swab is not acceptable for culture.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated7 days