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Unit Code 8628:
Galactokinase, Blood

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Specimen Required

Submit only 1 of the following specimens:

 

Heparinized whole blood must arrive within 48 hours of draw.

If specimen cannot arrive within 48 hours, please send washed

erythrocytes.

 

Whole Blood to be Washed at Mayo Medical Laboratories

Draw blood in a green-top (heparin) tube(s) from a fasting patient

(4 hour preferred, nonfasting acceptable), and send 5 mL

of heparinized whole blood refrigerated. Specimen cannot be frozen.

Note:      1.  Draw date is required on request form for processing.

                  2.  Include type of specimen sent (heparinized whole blood)

                       on request form.

 

If preferred, washed packed red blood cells will also be accepted

as follows:

 

Washed Erythrocytes

Draw blood in a green-top (heparin) tube(s) from a fasting patient

(4 hour preferred, nonfasting acceptable), and process 2 mL

of heparinized whole blood as follows:

1.   Immediately centrifuge for 10 minutes at 650 x G.

2.   Discard the plasma and buffy coat layers.

3.   Add a cold 0.9% saline solution to the erythrocytes (about 2

      times the volume of erythrocytes).

4.   Mix gently by inversion and centrifuge again for 10 minutes at

      650 x G.

5.   Remove and discard the saline.

6.   Repeat the wash steps (3-5) 2 more times.

7.   After the final centrifugation, remove and discard the saline

      and a thin layer of the top cells.

8.   Immediately freeze the washed, packed cells (red cell pellet

      from step 7), and send specimen frozen in plastic vial.

Note:      1.  Draw date is required on request form for processing.

                  2.  Include type of specimen sent (washed packed red blood

                       cells) on request form.

Transport Temperature

Varies                             
Refrig\Ambient NO\Frozen NO-Whole Blood
Frozen\Refrig NO\Ambient NO-Washed Packed Red Blood Cells

Reject Due To

Specimens Other Than:                 Whole blood or washed packed

                                                                red blood cells

Anticoagulants Other Than:           Heparin

Hemolysis:                                          Whole blood specimens cannot

                                                                be hemolyzed. Washed red

                                                                cells-acceptable.

Thawing:                                              NA

Lipemia:                                               No

Day(s) and Time(s) Test Performed

Tuesday; 9:00 a.m.

Key