Search our Test Catalog. Specify what to search (name, test code, titles, everywhere) and how to search (begins with, contains)

Unit Code 8599:
Uroporphyrinogen Decarboxylase (UPG D), Erythrocytes

Print Friendly View
Print Page Email Page

Specimen Required

All porphyrin tests on erythrocytes can be performed on 1 draw tube.

 

Submit only 1 of the following specimens:

     

Washed Erythrocytes

1. Have patient fast for 12 to 14 hours. Water may be taken as needed.

     No other liquids are allowed.

2. Patient should abstain from alcohol for 24 hours.

    Note:    Please include a list of medications the patient is currently

                     taking and forward with the specimen.

3. Draw a full, green-top (heparin) tube; and process entire

     heparinized whole blood specimen as follows:

     A.  Place on wet ice immediately.

     B.  Transfer to a 12-mL graduated centrifuge tube.

     C.  Centrifuge for 10 minutes at 2,000 rpm.

    D. Record volume of packed cells and the total volume of the

         specimen. This information must be sent with the specimen.

     E.  Discard supernatant plasma.

     F.   Wash erythrocytes 2 times by resuspension with 5 mL of cold 0.9%

           saline, discarding supernatant after each washing.

     G.  Resuspend packed cells to the original total volume of 5 mL with

           0.9% saline. Invert gently to mix.

4. Send washed erythrocyte suspension frozen in plastic vial.

    Note:   Volume of packed cells and total volume of  

                erythrocyte suspension (red cells saline) are

                required on request form for processing.

 

 

Whole Blood to be Washed at Mayo Medical Laboratories

Specimen must arrive within 48 hours of draw.

If specimen cannot arrive within 48 hours, please send washed

erythrocytes; and include the cell suspension information.

 

1. Have patient fast for 12 to 14 hours. Water may be taken as needed.

     No other liquids are allowed.

2. Patient should abstain from alcohol for 24 hours.

    Note:    Please include a list of medications the patient is currently

                     taking and forward with the specimen.  

3. Draw a full, green-top (heparin) tube; and send entire heparinized

     whole blood specimen refrigerated. Specimen cannot be frozen.

4.Test will be cancelled if frozen whole blood is received.

Transport Temperature

Varies

Frozen\Refrig NO\Ambient NO - Washed cells

Refrig\Frozen NO\Ambient NO - Whole blood

 

Reject Due To

Specimens Other Than:                 Erythrocyte suspension

                                                                or heparinized whole blood

Anticoagulants Other Than:           Heparin

Hemolysis:                                          Reject.

Thawing:                                              Warm Reject; Cold Ok;

                                                                Note to Lab

Lipemia:                                               N/A

Day(s) and Time(s) Test Performed

Monday, Wednesday, Friday; 8:00 A.M.

Key