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Test ID: CATP    
Catecholamine Fractionation, Plasma, Free

Specimen Type Describes the specimen type needed for testing

Plasma EDTA Meta

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Collection Container/Tube: Catecholamine tubes containing EDTA-sodium metabisulfite solution (Supply T066) (tubes have a 6-month expiration time)

Submission Container/Tube: Plastic vial

Specimen Volume: 3 mL

Collection Instructions:

1. Drawing from a catheter is required.

2. Unless the purpose of the measurement is drug monitoring, discontinue any epinephrine, norepinephrine, or dopamine injections or infusions for at least 12 hours before specimen draw.

3. Discontinue drugs that release epinephrine, norepinephrine, or dopamine or hinder their metabolism for at least 1 week before obtaining the specimen (see Cautions for details). If this is not possible for medical reasons, contact the laboratory and discuss whether a shorter drug withdrawal period may be possible in a particular case.

4. Do not perform the test on patients withdrawing from legal or illegal drugs known to cause rebound plasma catecholamine release during withdrawal (see Cautions for details).

5. The patient must refrain from eating, using tobacco, and drinking caffeinated beverages for at least 4 hours before the specimen is drawn.

6. Calm the patient by giving complete instructions and reassurance regarding the procedure.

7. Insert an indwelling intravenous catheter. Flush with 3 mL of NaCl, using positive pressure.

8. Have the patient rest for 30 minutes in the supine position in a quiet room.

9. At the end of the 30 minutes, withdraw and discard a minimum of 3 mL of blood to remove the saline out of the catheter.

10. If provocative sampling (eg, standing specimen) is required, perform provocative maneuver immediately after obtaining supine specimen. Obtain standing specimen immediately.

11. For each specimen, draw 10 mL of blood into the chilled EDTA-sodium metabisulfite 10-mL tube.

12. Specimens must remain at refrigerated temperature during processing and transport.

13. Separate plasma in a refrigerated centrifuge within 30 minutes of draw.

14. Freeze specimen immediately.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

2 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Plasma EDTA MetaFrozen7 days