Unit Code 8522:
Acetylcholinesterase, Erythrocytes
Specimen Required
Submit only 1 of the following specimens:
Whole Blood to be Washed at Mayo Medical Laboratories
Heparinized whole blood must arrive within 72 hours of draw.
Note: If specimen cannot arrive within 72 hours, please send washed
erythrocytes.
Draw blood in a green-top (heparin) tube(s) from a fasting patient
(4 hour preferred, nonfasting acceptable), and send 5 mL of heparinized
whole blood refrigerated. Specimen cannot be frozen.
Note: 1. Draw date is required on request form for processing.
2. Include type of specimen sent (heparinized whole blood)
on request form.
If preferred, washed packed red blood cells will also be accepted
as follows:
Washed Erythrocytes
Draw blood in a green-top (heparin) tube(s) from a fasting patient
(4 hour preferred, nonfasting acceptable), and process 2 mL
of heparinized whole blood as follows:
1. Immediately centrifuge for 10 minutes at 650 x G.
2. Discard the plasma and buffy coat layers.
3. Add a cold 0.9% saline solution to the erythrocytes (about 2
times the volume of erythrocytes).
4. Mix gently by inversion and centrifuge again for 10 minutes
at 650 x G.
5. Remove and discard the saline.
6. Repeat the wash steps (3-5) 2 more times.
7. After the final centrifugation, remove and discard the saline
and a thin layer of the top cells.
8. Immediately freeze the washed, packed cells (red cell pellet
from step 7), and send specimen frozen in a plastic vial.
Note: 1. Draw date is required on request form for processing.
2. Include type of specimen sent (washed packed red blood
cells) on request form.
Transport Temperature
Varies
Refrig\Ambient NO\Frozen NO - Whole Blood
Frozen\Refrig NO\Ambient NO - Washed Packed Red Blood Cells
Reject Due To
Specimens Other Than: Whole blood or washed packed
red blood cells
Anticoagulants Other Than: Heparin preferred, EDTA,
ACD also acceptable
Hemolysis: Whole blood specimens cannot
be hemolyzed. Washed red
cells-acceptable.
Thawing: Varies
Lipemia: No
Day(s) and Time(s) Test Performed
Monday, Thursday; 10:00 a.m.
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