BARRP - Specimen: Bartonella, Molecular Detection, PCR

Test Catalog

Test Name

Test ID: BARRP    
Bartonella, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Advisory Information

BART / Bartonella Antibody Panel, IgG and IgM, Serum and Warthin-Starry tissue stain (70012 / Pathology Consultation) should be considered if PCR is negative and there is a strong suspicion of disease caused by these organisms.

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Bartonella species DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Fresh tissue or biopsy

Sources: Heart valve, liver, lymph node, or spleen

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3)

Collection Instructions: Collect fresh tissue specimen.

Specimen Stability Information: Refrigerated (preferred) <7 days/ Frozen <7 days  

 

Specimen Type: Paraffin-embedded tissue block

Sources: Heart valve, liver, lymph node, or spleen

Container/Tube: Sterile vial

Specimen Volume:  Submit formalin-fixed paraffin-embedded tissue block to be cut and returned; or two to five 10-micron sections in a sterile container.

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Fluid

Sources: Cerebrospinal, synovial, or ocular (eg, vitreous humor fluid)

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated (preferred) <7 days/Frozen <7 days

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Fresh tissue or biopsy: 5 mm(3); Paraffin-embedded tissue block: two 10-micron sections; Ocular fluid: 0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Tissue in formalin, formaldehyde, or acetone; slides; bone marrow

 

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesVaries