HAEVP - Specimen: Hemolytic Anemia Evaluation

Test Catalog

Test ID: HAEVP    
Hemolytic Anemia Evaluation

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

This is a consultative evaluation in which the case will be evaluated and appropriate tests performed, at an additional charge, and the results interpreted.


Eosin-5-malemide (EMA) binding (band 3) flow cytometry will be performed on all cases.


Note: RBCE / Reflexed RBC Enzymes, Blood includes: adenosine deaminase, adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5' nucleotidase.

Specimen Type Describes the specimen type needed for testing

Whole Blood ACD-B
Whole Blood EDTA
Whole Blood Slide

Advisory Information

Two whole blood EDTA specimens, 2 whole blood ACD specimens, an EDTA control specimen, and 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol) are required for testing.

For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.

Shipping Instructions

Specimens must arrive within 72 hours of draw.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.


Specimen Type: Blood

Container/Tube: Lavender top (EDTA) and yellow top (ACD)

Specimen Volume:

EDTA: Two 4-mL vials

ACD: Two 6-mL vials

Collection Instructions:

1. Immediately refrigerate specimens after draw.

2. Send specimens in original tubes. Do not aliquot.

3. Prepare smears.

4. Rubber band patient specimen and control vial together.



Specimen Type: Slides

Container/Tube: Blood smears

Specimen Volume: 2 well-made peripheral blood smears

Collection Instructions: Collect 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol).


Shipping Normal Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Label clearly on outermost label normal control.

3. Immediately refrigerate specimen after draw.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together.


1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.

2. Hemolytic Anemia Patient Information Sheet (T705) is available in Special Instructions, please fill out for a more complete evaluation by the signing Hematopathologist

3. If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

EDTA Blood: 3 mL; ACD Blood: 5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross reject







Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
ControlRefrigerated72 hours
Whole Blood ACD-BRefrigerated72 hours
Whole Blood EDTARefrigerated72 hours
Whole Blood SlideRefrigerated