HAEVP - Specimen: Hemolytic Anemia Evaluation

Test Catalog

Test ID: HAEVP    
Hemolytic Anemia Evaluation

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

The most common RBC enzymes (G6PD / Glucose-6-Dehydrogenase (G-6-PD), Quantitative, Erythrocytes; PK / Pyruvate Kinase, Erythrocytes; GPI / Glucose Phosphate Isomerase, Erythrocytes; and HEXK / Hexokinase, Blood) will always be performed. If these are normal, the second tier enzymes will be performed (provided sufficient sample volume). If second tier enzymes are desired even if the first tier is abnormal, please fill out the Hemolytic Anemia Patient Information sheet (T705) and indicate this desire. HPLC, capillary electrophoresis, and hemoglobin stability studies will always be performed. Reflex testing required to identify a hemoglobin abnormality can be added as the case requires. Osmotic fragility and eosin-5-maleimide (EMA) binding (band 3) flow cytometry will be performed on all cases. A normal shipping control for osmotic fragility (OF) is necessary to exclude false positive results due to preanalytical artifact.

 

OF and EMA binding testing will be canceled if no shipping control is received or if the shipping control is abnormal.

 

This is a consultative evaluation in which the case will be evaluated and appropriate tests performed, at an additional charge, and the results interpreted. If a peripheral blood smear is provided, the morphologic features will be incorporated into the interpretation. If a Hemolytic Anemia Patient Information sheet (T705) is received with the sample, the reported clinical features or clinical impression will be incorporated into the interpretation.

 

For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.

 

Note: RBCE / Reflexed RBC Enzymes, Blood (second-tier enzymes) includes: adenosine deaminase, adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5' nucleotidase.

Specimen Type Describes the specimen type needed for testing

Control
Whole Blood ACD-B
Whole Blood EDTA
Whole Blood Slide

Advisory Information

Two whole blood EDTA specimens, 2 whole blood ACD specimens, an EDTA control specimen, and 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol) are required for testing.

For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.

Shipping Instructions

Specimens must arrive within 72 hours of draw.

Necessary Information

Two whole blood EDTA specimens, 2 whole blood ACD specimens, an EDTA control specimen, and 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol) are required for testing.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Additional Information

For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.

 

Patient:

Specimen Type: Blood

Container/Tube: Lavender top (EDTA) and yellow top (ACD)

Specimen Volume:

EDTA: Two 4-mL vials

ACD: Two 6-mL vials

Collection Instructions:

1. Immediately refrigerate specimens after draw.

2. Send specimens in original tubes. Do not aliquot.

3. Prepare smears.

4. Rubber band patient specimen and control vial together.

 

Patient:

Specimen Type: Slides

Container/Tube: Blood smears

Specimen Volume: 2 well-made peripheral blood smears

Collection Instructions: Collect 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol).

 

Shipping Normal Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Label clearly on outermost label normal control.

3. Immediately refrigerate specimen after draw.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together.

Forms

1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.

2. Hemolytic Anemia Patient Information (T705) in Special Instructions. Please fill out for a more complete evaluation by the signing Hematopathologist.

3. If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

EDTA Blood: 3 mL; ACD Blood: 5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
ControlRefrigerated72 hours
Whole Blood ACD-BRefrigerated72 hours
Whole Blood EDTARefrigerated72 hours
Whole Blood SlideRefrigerated
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