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Test ID: HV1CD    
HIV-1 and HIV-2 Antibodies for Cadaveric or Hemolyzed Specimens, Serum

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

This test begins with HIV-1/-2 antibody EIA. If HIV-1/-2 antibody EIA is reactive, then HIV-1/-2 antibody differentiation is performed at an additional charge.

HIV-1 and HIV-2 antibody differentiation test begins with the Bio-Rad Multispot HIV-1/HIV-2 Rapid Test, with the following reflex testing. In specimens with indeterminate HIV-1 antibody but negative HIV-2 antibody results, HIV-1 antibody confirmation by Western blot is performed at an additional charge. In specimens with reactive or unreadable results for both HIV-1 and HIV-2 antibodies, HIV-1 antibody confirmation by Western blot and HIV-2 antibody confirmation are performed at additional charges. In specimens with negative HIV-1 antibody, but reactive HIV-2 antibody results, HIV-2 antibody confirmation is performed at an additional charge.

 

This is the appropriate test for testing symptomatic individuals. 

 

This test is not appropriate for asymptomatic individuals. To screen for HIV antibodies in hemolyzed specimens from asymptomatic individuals with or without risk factors for HIV infection, order HV1CM / HIV-1 and HIV-2 Antibody Screen for Hemolyzed Specimens, Serum.

 

See HIV Testing Algorithm (Fourth Generation Screening Assay) Including Follow-up of Reactive HIV Rapid Serologic Test Results in Special Instructions.

Specimen Type Describes the specimen type needed for testing

Serum

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Collection Container/Tube: 

Preferred: Serum gel

Acceptable: Red top    

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Spin down and remove serum from clot within 24 hours.

Additional Information:

1. If the initial EIA result is negative and this test was ordered as a follow-up evaluation of a specimen with a reactive rapid HIV antibody test result, clients must call Mayo Medical Laboratories at 800-533-1710 or 507-266-5700 to add supplemental testing HIV-1/-2 antibody differentiation by membrane immunoconcentration (HIVDI).

2. The HV1WB / HIV-1 Antibody Confirmation by Western Blot, Serum test has not been FDA-approved for testing on cadaveric specimens; if performed, testing will be resulted with a disclaimer.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
SerumFrozen (preferred)30 days
 Ambient 7 days
 Refrigerated 7 days

Key