BUAUC - Specimen: Busulfan, Intravenous Dose, Area Under the Curve (AUC), Plasma

Test Catalog

Test ID: BUAUC    
Busulfan, Intravenous Dose, Area Under the Curve (AUC), Plasma

Specimen Type Describes the specimen type needed for testing

Plasma Na Heparin

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Four plasma specimens with different draw times (keep all specimens under 1 order) and a completed Busulfan Information: Mail-In Specimen Instructions (T559) are required.


Collection Container/Tube: Green top (sodium heparin)

Submission Container/Tube: Plastic vials

Specimen Volume: 1 mL

Collection Instructions: 

1. The first specimen should be drawn immediately after termination of intravenous infusion of 0.8 mg/kg busulfan.

2. Additional specimens should also be drawn at 1 hour, 2 hours, and 4 hours after termination of infusion.

3. Label each specimen appropriately (exact time of draw).

4. Busulfan degrades quickly at room temperature. Each specimen should be kept in wet ice slurry or refrigerated at 4 degrees C. Specimen should be centrifuged as soon as possible after collection. Separate the plasma from each specimen and transfer it to individual 5-mL plastic tubes. Immediately freeze these plasma specimens at -20 degrees C.

Forms: Busulfan Information: Mail-In Specimen Instructions (T559) in Special Instructions

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.25 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross OK


Mild OK; Gross OK


Mild OK; Gross OK


Serum gel tube

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Plasma Na HeparinFrozen (preferred)28 days
 Refrigerated 72 hours
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