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Test ID: CHIDB    
Chimerism-Donor

Available on the App Store

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Complete chimerism analysis also requires submission of CHRGB / Chimerism-Recipient Germline (Pre) and CHEP / Chimerism-Recipient Engraftment (Post) specimens. These tests must be ordered on both the pre and post specimens under separate order numbers. The 3 specimens do not need to be submitted at the same time.

 

Forms:

1. Chimerism Analysis Information Sheet (Supply T594) in Special Instructions.

2. If not ordering electronically, submit a Hematopathology/Molecular Oncology Request Form (Supply T241) with the specimen.

 

The following information is required:

1. Pertinent clinical history

2. Specimen source

3. Donor identifier and donor date of birth

4. Donor date of collection

 

Specimen must arrive within 168 hours of collection.

 

Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: ACD

Specimen Volume: 6 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: ACD

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

 

Specimen Type: Buccal swab

Container/Tube: Buccal Smear Collection Kit (Supply T543)

Specimen Volume: 2 Cyto-Pak brushes-1 per cheek

Collection Instructions:

1. Patient should rinse out mouth vigorously with mouthwash for approximately 15 seconds.

2. Remove Cyto-Pak brush from container only touching "stick" end. Save container.

3. Using medium pressure, rotate brush several times on inside of cheek.

4. Return brush to container and cap.

5. Repeat steps 2 through 4 on other cheek using second brush.

6. It is important that patient's buccal cells are not contaminated with cells from any other source. Do not touch bristles. Do not brush too vigorously. If blood appears, discard brush. Restart collection process.

7. Label each container with patient's name and order number or hospital/clinic number.

Additional Information: It is important that the cells do not dry out during shipping. Ensure that container is tightly sealed.

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Blood: 3 mL/Bone Marrow: 2 mL/Lesser volumes may be acceptable, depending on white cell count. Call Mayo Medical Laboratories at 800-533-1710 or 507-266-5700 with questions.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesAmbient (preferred)7 days
 Refrigerated 7 days