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Test ID: PROCT    
Prolonged Clot Time Profile

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

Initial testing includes: prothrombin time (PT), activated partial thromboplastin time (APTT), dilute Russells viper venom time (DRVVT), thrombin time (bovine), fibrinogen, D-dimer, soluble fibrin monomer, and special coagulation interpretation.
If PT is > or =14 seconds, PT mix will be performed.
If APTT is >36 seconds, APTT mix will be performed.
If APTT mix is >36 seconds with no evidence of heparin in samples, platelet neutralization procedure will be performed.
If DRVVT ratio is > or =1.2, DRVVT mix and DRVVT confirmation will be performed.
If thrombin time is >23 seconds, reptilase time will be performed.

If PT, APTT, or DRVVT is prolonged, coagulation factor assays may be performed.

Specimen Type Describes the specimen type needed for testing

Plasma Na Cit

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

See Coagulation Studies in Special Instructions.

 

Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 5 mL in 5 plastic vials each containing 1 mL

Collection Instructions:

1. Spin down, remove plasma, and spin plasma again.

2. Freeze specimens immediately at < or =-40 degrees C, if possible.

3. Send specimens in the same shipping container.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. If priority specimen, mark request form, give reason, and request a call-back.

3. Patient should not be receiving Coumadin or heparin.

Forms: Coagulation Patient Information Sheet (Supply T675) in Special Instructions

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

4 mL in 4 plastic vials each containing 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Plasma Na CitFrozen14 days

Key