BDIAL - Specimen: Bleeding Diathesis Profile, Limited

Test Catalog

Test ID: BDIAL    
Bleeding Diathesis Profile, Limited

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

Initial testing includes: prothrombin time (PT), activated partial thromboplastin time, thrombin time (bovine), fibrinogen, D-dimer, soluble fibrin monomer, coagulation factor VIII activity assay, von Willebrand factor antigen, von Willebrand factor activity, factor XIII screen, and special coagulation interpretation.  

If PT is > or =14 seconds, PT mix will be performed.

If APTT is >36 seconds, APTT mix and DRVVT will be performed.

If DRVVT ratio is > or =1.2, DRVVT mix will be performed.

If APTT mix is >36 seconds with no evidence of heparin in samples, platelet neutralization procedure will be performed.

If DRVVT ratio is > or =1.2, DRVVT mix and DRVVT confirmation will be performed.

If von Willebrand factor activity assay is <55%, von Willebrand factor ristocetin cofactor activity assay will be performed.  

If von Willebrand factor (VWF) antigen is <55%, the VWF activity is <55%, or the VWF activity:VWF antigen ratio is <0.8, VWF multimer analysis will be performed.

If appropriate, coagulation factor assays or Staclot LA will be performed at an additional charge to clarify significant abnormalities in the screening clotting times.

If factor VIII result is <55%, the factor VIII inhibitor screen may be performed along with the Bethesda titering assay, if inhibitor screen is positive.

 

See Hemophilia Testing Algorithm in Special Instructions.

Specimen Type Describes the specimen type needed for testing

Plasma Na Cit

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

See Coagulation Studies in Special Instructions.

 

Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 5 mL in 5 plastic vials each containing 1 mL

Collection Instructions:

1. Spin down, remove plasma, and spin plasma again.

2. Freeze specimens immediately at < or =-40 degrees C, if possible.

3. Send specimens in the same shipping container.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Patient should not be receiving Coumadin or heparin.

3. If priority specimen, mark request form, give reason, and request a call-back.

Forms:

1. Coagulation Patient Information Sheet (T675)

2. If not ordering electronically, complete, print, and send a Coagulation Test Request Form (T753) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/coagulation-test-request-form.pdf)

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

Internal:
Two 4.5 mL 3.2% Sodium Citrate
External:
4 mL in 4 plastic vials, 1 mL each

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Plasma Na CitFrozen14 days