Specimen Source Identification
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Due to the complex nature of this test, direct communication (eg, pathologist to pathologist) is required to avoid delays in sample processing and ensure an understanding of relevant case details.
A brief letter that includes the following 4 pieces of information is required for all orders:
1. Reason for testing, including detailed information regarding what specific comparisons are requested.
2. Clear identification of the known and unknown specimens.
3. Copies of all existing pathology reports pertaining to submitted issue specimens.
4. Contact information for the ordering physician.
1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions.
2. If not ordering electronically, submit a Molecular Genetics Request Form (Supply T245) with the specimen.
Chain of custody documentation is not available. This test is not intended for medico-legal or forensic purposes.
Specimen must arrive within 96 hours of draw.
Specimen Type: Whole blood
Preferred: Lavender top (EDTA) or yellow top (ACD)
Acceptable: Any anticoagulant
Specimen Volume: 3 mL
1. Invert several times to mix blood.
2. Send specimen in original tube.
Specimen Stability Information: Ambient (preferred)/Refrigerated
Specimen Type: Tissue block or slide
1. Submit formalin-fixed, paraffin-embedded (FFPE) tissue block (preferred) or 4 to 10 unstained sections (each 5 micron-thick) plus 1 slide stained with hematoxylin and eosin (H and E).
2. The number of unstained sections required depends on the amount of tissue that can be used for analysis.
3. For very small tissue fragments, 10 sections are recommended; for large tissue fragments, 4 sections are generally sufficient.
4. If known and unknown specimens are within the same block, include labeled H and E-stained slide identifying the known and unknown specimens.
5. Specimen ID tests involving very small fragments of tissue, including most floaters are performed at the discretion of the reviewing pathologist. Cases involving floaters are usually rejected due to an insufficient amount of the floater tissue.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Blood: 0.5 mL/Tissue: send paraffin-embedded whole tissue OR 4x5 micron-thick sections plus 1 H/E slide
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.