HPFH - Specimen: Hemoglobin F, Red Cell Distribution, Blood

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Test ID: HPFH    
Hemoglobin F, Red Cell Distribution, Blood

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

The percent of the patient’s hemoglobin F is very important to the clinical utility of this test. HBF / Hemoglobin F, Blood will be performed at an additional charge to determine if Hemoglobin F, Red Cell Distribution testing is warranted. If the hemoglobin F value from HBF / Hemoglobin F, Blood are normal for age or if value is <5% the HPFH / Hemoglobin F, Red Cell Distribution, Blood will not be performed. Samples with hemoglobin F (Hb F) levels >35% will be accepted but are discouraged due to decreased test specificity. For these markedly elevated Hb F levels, a hemoglobin electrophoresis profile with request for beta globin gene molecular testing for elevated Hb F levels is recommended.

Specimen Type Describes the specimen type needed for testing

Whole Blood EDTA

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Lavender top (EDTA)
Specimen Volume:
3 mL
Additional Information:
1.
Patient's age and sex are required.
2. Indicate the percent of hemoglobin F detected by hemoglobin electrophoresis. If this is not indicated, we will perform HBF / Hemoglobin F, Blood.

3. Indicate the reason for testing.
4. For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.

Forms: If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild reject; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
Whole Blood EDTARefrigerated7 days

Key