Amyloid Protein Identification, Paraffin, LC-MS/MS
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
In all cases with adequate tissue, an initial Congo red stain is performed before mass spectrometry testing to confirm positivity and pattern of amyloid deposition can be considered when interpreting mass spectrometry results. If initial Congo red stain is positive for amyloid, the 89054 / Congo Red, Stain will always be billed at an additional charge. If initial Congo red stain is negative for amyloid, the 82091 / Amyloid Protein Identification, Paraffin, LC-MS/MS will be cancelled and 9901 / Puchtler's Modification of Bennhold's Stain for Amyloid (Congo Red) will be charged. A pathology consultation is typically not required. If the amyloid subtyping results do not fit the clinical findings, a 70012 / Pathology Consultation may be added if appropriate, upon client approval.
See Laboratory Approach to the Diagnosis of Amyloidosis in Special Instructions
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen Type: Formalin-fixed or B5-fixed, paraffin-embedded tissue block
Container/Tube: Pathology Packaging Kit (T554)
1. Do not send fixed tissue slides. Testing can only be done on paraffin-embedded tissue blocks.
2. Attach the green pathology address label included in the kit to the outside of the transport container.
1. Preliminary pathology report and history are required.
2. A brief explanatory note or consultative letter is also recommended.
Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
Cardiovascular Test Request Form (T724) (http://www.mayomedicallaboratories.com/it-mmfiles/cardiovascular-request-form.pdf)
Hematopathology/Cytogenetics Test Request Form (T726) (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf)
Fixed tissue slides
Nonformalin fixed tissue
Nonparaffin embedded tissue
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
The Amyloid Protein Identification test employs Liquid Tissue® sample preparation licensed from Expression Pathology Inc., Rockville MD, under U.S. Patent 7,473,532 and patents pending and foreign equivalents thereof.