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Test Catalog

Test ID: PSAFT    
Prostate-Specific Antigen (PSA), Total and Free, Serum

Specimen Type Describes the specimen type needed for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Collection Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Spin down within 3 hours of draw and separate serum from cells.

Additional Information:

1. Free prostate-specific antigen (PSA) can only be added on within 12 hours of performing total PSA. Specimen must have been shipped frozen.

2. Include patient’s age.

Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

General Request Form (T239) (

Oncology Test Request Form (T729) (

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild OK; Gross reject


Mild OK; Gross OK





Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
SerumFrozen90 days