Test ID: PNRP
Pneumocystis jiroveci, Molecular Detection, PCR
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Pneumocystis species DNA is not likely.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Acceptable Specimens: Cerebrospinal fluid, respiratory specimens (eg, bronchoalveolar lavage fluid, bronchial washing, sputum), pleural fluid, respiratory tissue (fresh)
Unacceptable Specimens: Body fluid other than cerebrospinal fluid or pleural fluid, blood, bone, non-respiratory tissue, bone marrow, organ tissues other than lung, paraffin-embedded tissue, specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth), stool, swab, tissue in formalin fluid, urine, specimen >7 days old
Specimen must arrive within 7 days of collection; specimen >7 days will be rejected.
Specimen source is required.
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Cerebrospinal or pleural
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Respiratory
Sources: Bronchoalveolar lavage fluid, bronchial washing, or sputum
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Tissue
Sources: Respiratory
Container/Tube: Sterile container
Specimen Volume: 5-10 mm
Collection Instructions: Submit fresh tissue.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Body fluid other than cerebrospinal fluid or pleural fluid, blood, bone, non-respiratory tissue, bone marrow, organ tissues other than lung, paraffin-embedded tissue, specimen in anaerobe vial or viral transport medium, stool, swab, tissue in formalin fluid, urine, specimen >7 days old |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
External
link
PDF
document
Excel
document
Word
document