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Test ID: PNRP    
Pneumocystis jiroveci, Molecular Detection, PCR

Specimen Type Describes the specimen type needed for testing

Varies

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Pneumocystis species DNA is not likely.

 

Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.

 

Preferred Specimens: Cerebrospinal fluid (CSF), respiratory specimens (eg, bronchoalveolar lavage fluid, bronchial washing, sputum), pleural fluid, respiratory tissue (fresh)

 

Acceptable Specimens: If no fresh specimen is available, digested respiratory specimens treated with N-acetyl-L-cysteine (NALC)/NaOH are acceptable (eg, bronchoalveolar lavage [BAL] fluid, bronchial washing, respiratory fluid, sputum, or tracheal secretion), as are NALC/NaOH-treated gastric washes. 

 

Unacceptable Specimens: Body fluid other than CSF or pleural fluid, blood, bone, nonrespiratory tissue, bone marrow, organ tissues other than lung, paraffin-embedded tissue, specimen in anaerobe vial or viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth), stool, swab, tissue in formalin fluid, urine, specimen >7 days old

 

Specimen must arrive within 7 days of collection; specimen >7 days will be rejected.

 

Specimen source is required.

 

Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: CSF or pleural

Container/Tube: Sterile container

Specimen Volume: 1 mL

                                                          

Specimen Type: Respiratory

Sources: BAL, bronchial washing, or sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Tissue

Sources: Respiratory

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions: Submit fresh tissue.

 

Acceptable:

NALC/NaOH-digested specimen must arrive within 7 days of digestion.

                                                               

Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, gastric washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

1. Submit digested specimen treated with NALC/NaOH.

2. Clearly indicate on container and order form that specimen is a digested specimen.

Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 7 days/Frozen 7 days

Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Body fluid other than CSF or pleural fluid, blood, bone, non-respiratory tissue, bone marrow, organ tissues other than lung, paraffin-embedded tissue, specimen in anaerobe vial or viral transport medium, stool, swab, tissue in formalin fluid, urine, specimen >7 days old

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Frozen 7 days