Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
See Hyperoxaluria Diagnostic Algorithm in Special Instructions.
Specimen Type Describes the specimen type needed for testing
Plasma Na Heparin
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Any client who has never drawn a specimen for this test should call 800-533-1710 or 507-266-5700 and ask for the Renal Laboratory for more detailed instructions.
1. Fasting (12 hours)
2. Patient should avoid taking vitamin C supplements for 24 hours prior to draw.
Specimen Type: Acidified plasma
Collection Container/Tube: Green top (sodium heparin)
Submission Container/Tube: Plastic vial
Specimen Volume: 5 mL
1. Place on wet ice immediately.
2. Centrifuge for 10 minutes at 3,500 rpm at 4 degrees C within 1 hour of draw.
3. Adjust the pH of the plasma specimen to a pH of 1 to 3.5 (ideal range is 2.3-2.7) with approximately 10 mcL concentrated (12N) HCl per 1 mL plasma.
Additional Information: Nonacidified specimen can be accepted if the heparinized plasma is properly frozen. However, a disclaimer will be added in nonacidified plasma, oxalate values may increase spontaneously (average 50% increase for plasma oxalate <15 mcmol/L; average 10% increase for plasma oxalate >15 mcmol/L).
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Plasma Na Heparin||Frozen||14 days|