Test ID: LCMV
Cytomegalovirus (CMV), Molecular Detection, PCR
Specimen Type 
Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen source is required.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Body or ocular fluid
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Container/Tube: Sterile container
Specimen Volume: 1.5 mL
Specimen Stability Information: Refrigerated 7 days
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days
Additional Information:
1. The high sensitivity of amplification by PCR requires the specimen be processed in an environment in which contamination of the specimen by CMV DNA is not likely.
2. Instructions for aliquoting CSF for PCR testing:
-All aliquoting for PCR testing should be done under a hood that has been bleached before and after use.
-Aliquot only 1 patient at a time.
-Use sterile (individually wrapped) plastic transfer pipettes.
-If aliquoting into several tubes from 1 patient tube, dedicate the first tube to PCR testing. Cap immediately after transferring specimen.
-Always dedicate 1 tube to PCR testing.
Specimen Type: Swab
Sources:
Preferred: Dermal, genital, eye, or throat
Acceptable: Urethral
Container/Tube: Culture transport swab
Specimen Volume: Entire specimen
Collection Instructions: Place swab back into swab cylinder.
Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, etc.
Container/Tube: Sterile container with 1 to 2 mL of sterile saline or multi-microbe medium (M5) (Supply T484)
Specimen Volume: Entire collection
Collection Instructions: Collect a fresh tissue specimen.
Specimen Stability Information: Refrigerated 7 days
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 2 mL
Collection Instructions: Collect a random urine specimen.
Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days
Acceptable:
Specimen Type: Amniotic fluid
Container/Tube: Amniotic fluid container
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days
Specimen Type: Bone marrow
Container/Tube: Sterile container or lavender top (EDTA)
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated 7 days
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Calcium alginate-tipped swab, wood swab, or transport swab containing gel |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |
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